Analysis of amantadine in Laminaria Japonica and seawater of Daqin Island by ultra high performance liquid chromatography with positive electrospray ionization tandem mass spectrometry

• Published in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2019-09, Vol.1126-1127, p.121697, Article 121697
• Main Authors: Xu, Yingjiang, Ren, Chuanbo, Han, Dianfeng, Gong, Xianghong, Zhang, Xiuzhen, Huang, Hui, Jiang, Fang, Cui, Yanmei, Zheng, Weiyun, Tian, Xiuhui
• Format: Article
• Language: English
• Subjects: Amantadine; Amantadine - analysis; Bioaccumulation factor; Chromatography, High Pressure Liquid - methods; Laminaria - chemistry; Laminaria Japonica; Limit of Detection; Linear Models; Reproducibility of Results; Seawater; Seawater - chemistry; Spectrometry, Mass, Electrospray Ionization - methods; Tandem Mass Spectrometry - methods; UHPLC-ESI-MS/MS; Water Pollutants, Chemical - analysis
• ISSN: 1570-0232; 1873-376X
• DOI: 10.1016/j.jchromb.2019.06.024

A sensitive and validated method for determination of amantadine in Laminaria Japonica and seawater was established using ultra high performance liquid chromatography with positive electrospray ionization tandem spectrometry (UHPLC-ESI-MS/MS). Laminaria Japonica was extracted with acetonitrile containing formic acid (1%), then purified with 10.0 g anhydrous sodium sulfate, 0.50 g C18 and 0.50 g PSA powder. Seawater added 10.0 mL 0.20 mol/L hydrochloric acid was purified with MCX solid phase extraction (SPE) cartridge. After extraction and purification, the supernatant of Laminaria Japonica and eluate of seawater were evaporated to nearly dry under a gentle stream of nitrogen at 40 °C. Acetonitrile-0.1% formic acid in water (3/7, v/v) was adjusted to 1.00 mL final volume. An aliquot (10 μL) was injected into the C18 column for separation with the mobile phase of acetonitrile and 0.1% formic acid in water at 0.25 mL·min−1. Calibration curves were linear ranged from 1.00 ng/mL to 20.0 ng/mL. Mean recoveries were 73.5% to 95.8%, and limit of detection (LOD) and quantification (LOQ) were 0.50 μg/kg and 1.00 μg/kg for Laminaria Japonica. Mean recoveries were 75.8% to 93.4%, and LOD and LOQ were 0.50 ng/L and 1.00 ng/L for seawater. Based on the method above, Laminaria Japonica and seawater in Daqin Island were analyzed in February to June continuously, lgBAF3.71 (bioaccumulation factor), indicating a bioenrichment effect. •No method for determination of Amantadine residue has been established in Laminaria Japonica and seawater.•UPLC-MS/MS has a single run time of 7.0 min per sample, economical of time, reagent and energy.•Our team is the first to monitor the pollution level of Amantadine in Laminaria Japonica and seawater.•The thesis is to providing the first characterization of Amantadine distribution in Laminaria Japonica comprehensively.