Monolithic mixed matrix membrane based on polyethersulfone/functionalized MWCNTs nanocomposite as an SPME fiber: Application to extract chlorophenols from human urine and serum samples followed by GC-ECD

• Published in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2020-08, Vol.1150, p.122190, Article 122190
• Main Authors: Matin, Amir Abbas, Biparva, Pourya, Gheshlaghi, Mohammad, Khosrowshahi, Elnaz Marzi, Farhadi, Khalil
• Format: Article
• Language: English
• Subjects: Chlorophenols; Chlorophenols - blood; Chlorophenols - isolation & purification; Chlorophenols - urine; Chromatography, Gas - methods; Gas chromatography; Humans; Limit of Detection; Linear Models; Mixed matrix membranes; Monolithic fiber; Nanocomposites - chemistry; Nanotubes, Carbon - chemistry; Polymers - chemistry; Reproducibility of Results; Solid phase microextraction; Solid Phase Microextraction - methods; Sulfones - chemistry
• ISSN: 1570-0232; 1873-376X
• DOI: 10.1016/j.jchromb.2020.122190

•A monolithic mixed matrix membrane as an SPME fiber was introduced.•The design of novel fibers provides a new perspective for the method development.•MWCNTs as sorbent and polyethersulfone as membrane were used in the fiber structure.•The application of developed fiber for the extraction of CPs from real samples was investigated.•The method showed good figure merits like low LOD, wide linear range and good repeatability. A monolithic mixed matrix membrane of functionalized multi-walled carbon nanotubes-polyethersulfone (MWCNT/PES) was prepared in a non-covalent approach and employed as an SPME fiber for extraction of chlorophenols (CPs). The proposed extraction method was followed by GC-ECD to determine the analytes. The influencing factors on the extraction efficiency such as pH, ionic strength, extraction and desorption temperature and time were studied. Under the selected conditions, calibration curves were linear over a wide concentration range from 0.005 to 1000 µgL−1 (r2 > 0.9961) for target analytes. In addition, the limits of detection (LOD) of the method were obtained in the range of 0.3–30 ng L−1. The relative standard deviation (RSD) for single fiber repeatability (n = 5) is from 1.4 to 4.6%. Fiber-to-fiber repeatability (n = 3) was also evaluated and the RSD is in the range of 1.3–6.3%. Applications of proposed fiber for extraction of CPs from the headspace of urine and serum samples were successfully investigated. The relative recovery in the biological samples spiked with different levels of CPs were in the range of 91.6–102.5%.