Optimization of cultivation conditions for fungichromin production from Streptomyces padanus PMS-702

A novel strain, Streptomyces padanus PMS-702, was employed to produce fungichromin (FC, a polyene macrolide antibiotic) in a shake flask cultivation. In comparing the effects of various carbon and nitrogen sources on PMS-702 cultivation, it was found that glucose and soybean meal medium yielded the...

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Bibliographic Details
Published in:Journal of the Chinese Institute of Chemical Engineers 2008, Vol.39 (1), p.67-73
Main Authors: Wu, Jiun-Yan, Huang, Jenn-Wen, Shih, Hsin-Der, Lin, Wei-Chen, Liu, Yung-Chuan
Format: Article
Language:English
Subjects:
Fungichromin
Optimization
Polyene macrolide antibiotic
Response surface methodology
Streptomyces padanus PMS-702
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Summary:A novel strain, Streptomyces padanus PMS-702, was employed to produce fungichromin (FC, a polyene macrolide antibiotic) in a shake flask cultivation. In comparing the effects of various carbon and nitrogen sources on PMS-702 cultivation, it was found that glucose and soybean meal medium yielded the highest FC within 2 days. Factors such as medium composition, cultivation temperatures, and initial pH were optimized for FC production with response surface methodology (RSM). The optimal cultural condition obtained is as follows: glucose 11.2 g/L, soybean meal 11.2 g/L, CaCO 3 0.46 g/L, temperature 31.7 °C, and an initial pH 5.5. Under these conditions, FC production reached 112 mg/L, about an increase of 2.86 times, as compared to results under basic conditions.
ISSN:0368-1653
DOI:10.1016/j.jcice.2007.11.006