Synthetic and biological identities of polymeric nanoparticles influencing the cellular delivery: An immunological link

[Display omitted] Enhanced understanding of bio-nano interaction requires recognition of hidden factors such as protein corona, a layer of adsorbed protein around nano-systems. This study compares the biological identity and fingerprint profile of adsorbed proteins on PLGA-based nanoparticles throug...

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Bibliographic Details
Published in:Journal of colloid and interface science 2019-11, Vol.556, p.476-491
Main Authors: Rezaei, Ghassem, Daghighi, Seyed Mojtaba, Raoufi, Mohammad, Esfandyari-Manesh, Mehdi, Rahimifard, Mahban, Mobarakeh, Vahid Iranpur, Kamalzare, Sara, Ghahremani, Mohammad Hossein, Atyabi, Fatemeh, Abdollahi, Mohammad, Rezaee, Farhad, Dinarvand, Rassoul
Format: Article
Language:English
Subjects:
Animals
Cellular uptake
CHO Cells
Cricetulus
Delivery
Drug Delivery Systems
Dysopsonin/opsonin ratio
Fc receptor
Humans
Mice
Nanoparticles - chemistry
Opsonin Proteins - chemistry
Opsonin Proteins - immunology
Particle Size
PLGA-based NPs
Protein corona
Protein Corona - chemistry
Protein Corona - immunology
RAW 264.7 Cells
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Summary:[Display omitted] Enhanced understanding of bio-nano interaction requires recognition of hidden factors such as protein corona, a layer of adsorbed protein around nano-systems. This study compares the biological identity and fingerprint profile of adsorbed proteins on PLGA-based nanoparticles through nano-liquid chromatography-tandem mass spectrometry. The total proteins identified in the corona of nanoparticles (NPs) with different in size, charge and compositions were classified based on molecular mass, isoelectric point and protein function. A higher abundance of complement proteins was observed in modified NPs with an increased size, while NPs with a positive surface charge exhibited the minimum adsorption for immunoglobulin proteins. A correlation of dysopsonin/opsonin ratio was found with cellular uptake of NPs exposed to two positive and negative Fc receptor cell lines. Although the higher abundance of dysopsonins such as apolipoproteins may cover the active sites of opsonins causing a lower uptake, the correlation of adsorbed dysopsonin/opsonin proteins on the NPs surface has an opposite trend with the intensity of cell uptake. Despite the reduced uptake of corona-coated NPs in comparison with pristine NPs, the dysopsonin/opsonin ratio controlled by the physicochemistry properties of NPs could potentially be used to tune up the cellular delivery of polymeric NPs.
ISSN:0021-9797
1095-7103
DOI:10.1016/j.jcis.2019.08.060