Mitigating effects of osmolytes on the interactions between nanoparticles and supported lipid bilayer

[Display omitted] To maintain osmotic balance, cells usually produce neutral solutes (i.e., osmolytes), together with charged species to cope with salinity stress. Osmolytes are known to be important in stabilizing/destabilizing macromolecules (e.g., proteins) via depletion /accumulation around thei...

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Bibliographic Details
Published in:Journal of colloid and interface science 2020-05, Vol.568, p.1-7
Main Authors: Xia, Zehui, Lau, Boris L.T.
Format: Article
Language:English
Subjects:
Chaotrope
Hofmeister series
Kosmotrope
Lipid Bilayers - chemistry
Membrane disruption
Microscale thermophoresis
Molecular Structure
Nanoparticles - chemistry
Osmotic Pressure
Particle Size
Phosphatidylcholines - chemistry
Quartz Crystal Microbalance Techniques
Quartz crystal microbalance with dissipation monitoring
Surface Properties
Trimethylamine N-oxide
Urea
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Summary:[Display omitted] To maintain osmotic balance, cells usually produce neutral solutes (i.e., osmolytes), together with charged species to cope with salinity stress. Osmolytes are known to be important in stabilizing/destabilizing macromolecules (e.g., proteins) via depletion /accumulation around their surfaces. To better understand the physiological fate of nanoparticles (NPs), we investigated the effect of osmolytes [(urea and trimethylamine N-oxide (TMAO)] and specific anions (NO3- and F-) on the interactions between NPs and supported lipid bilayers (SLBs). Carboxylated polystyrene NPs (60 nm) and 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) were chosen as model NPs and lipid. Quartz crystal microbalance with dissipation monitoring (QCM-D) was used to quantify NP deposition dynamics. Microscale thermophoresis (MST) was used to characterize the affinity between DOPC vesicles (or NPs) and osmolytes. Our results show that osmolytes are capable of protecting SLBs from NP-induced disruption. Upon NP deposition onto supported vesicle layers (SVLs), the leakage of encapsulated dyes decreased with the addition of osmolytes. The combination of kosmotropes (TMAO and F-) are more efficient than that of chaotropes (urea and NO3-) in weakening the hydrophobic interaction between NPs and SLBs by preferential binding to NPs and/or SLBs.
ISSN:0021-9797
1095-7103
DOI:10.1016/j.jcis.2020.02.041