Bacterial biofilm or biofouling networks with numerous resilience factors from real water supplies of Chennai and their enhanced susceptibility to biocompatible nanoparticles

• Published in: Journal of cleaner production 2019-09, Vol.231, p.872-898
• Main Authors: Karthick Raja Namasivayam, S., Francis, A.L., Arvind Bharani, R.S., Nachiyar, C.Valli
• Format: Article
• Language: English
• Subjects: Biofilm; Coliforms; Nanoparticles; Water quality; Water reservoirs
• ISSN: 0959-6526; 1879-1786
• DOI: 10.1016/j.jclepro.2019.05.199

In the present investigation, enhanced susceptibility of bacterial biofilm networks associated with water repositories of Chennai to silver and silica nanoparticles has been proposed. A total of 120 water samples were collected amid four seasons according to the standard methods and the collected samples were investigated for heterotrophic microorganisms, fecal coliforms and biofilm or biofouling. Bacterial strains segregated from the separate lake were assessed for biofilm or biofouling forming efficacy and their resilience to substantial metal, salt, various antibiotics. Dominant part of the bacterial strains confined from the respective reservoir showed biofilm development and high rate of biofilm framing adequacy was seen in Poondi Lake. E.coli (30–40%),Proteus sp (20–25%), Salmonella sp (10–20%) Pseudomonas aeruginosa (5–10%) and Lactobacillus (5%) sp were the major bacterial strains associated with biofilm of all the tested reservoirs. Bacterial strains related with biofilm of particular tried supplies demonstrated high resilience to heavy metal, salt and various antibiotics. High rate of multiple drug resistance was recorded in Poondi lake isolates (90%) followed by Porur (85%) and Chembarambakkam (81%).Vulnerability of the biofilm networks to nanoparticles like silver and silica was studied. Silver nanoparticles were orchestrated from leaf extrcat of Vitex negundo and silica from block powder separately which yield high stable, monodispersed particles.Both the nanoparticles realized viable restraint of the biofilm as concentration dependent manner. Be that as it may, noteworthy impact was recorded in silver nanoparticles treatment. Nanoparticles susceptibility testing against E.coli- Predominant member (65%) of the biofilm of all the reservior suggest that silver nanoparticles brought about distinct effect on biofilm networks which exhibited 99.0% of biofilm inhibition and improved spillage of protein, nucleic acid content.The study will give awareness about the sanitation and safety of water in the respective reservoirs and the enhanced susceptibility of the biofouling to the nanoparticles would suggest the possible utilization of nanotechnology principles as an effective green strategy in water treatment process [Display omitted] •Bacterial biofilm or biofouling associated with water reservoirs of Chennai was isolated by culture dependent methods.•Biofouling bacterial communities exhibited high rate of resilience to heavy metal, salt and multiple