Deep eutectic systems for carbonic anhydrase extraction from microalgae biomass to improve carbon dioxide solubilization

This work is the first proof-of-concept of the use of carbonic anhydrase (CA) enzyme from microalgae biomass, extracted with deep eutectic systems (DES), with the goal of engineering a solution that will lead to a breakthrough in the Carbon Capture and Utilization (CCU) strategy. Three distinct micr...

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Bibliographic Details
Published in:Journal of CO2 utilization 2022-11, Vol.65, p.102225, Article 102225
Main Authors: Craveiro, Rita, Dusschooten, Fleur, Nabais, Ana R., Boboescu, Iulian, Lo, Calvin, Neves, Luísa A., Sá, Marta
Format: Article
Language:English
Subjects:
Carbonic Anhydrase
Chlorella vulgaris
CO2 capture and utilization
Deep Eutectic System
Spirulina sp
Tisochrysis lutea
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Summary:This work is the first proof-of-concept of the use of carbonic anhydrase (CA) enzyme from microalgae biomass, extracted with deep eutectic systems (DES), with the goal of engineering a solution that will lead to a breakthrough in the Carbon Capture and Utilization (CCU) strategy. Three distinct microalgae were processed – Tisochrysis lutea, Chlorella vulgaris, and Spirulina sp. – with three DES – Choline chloride-Urea (ChCl-U), Choline chloride-Poly(ethylene glycol) (ChCl-PEG), and Poly(ethylene glycol)-Urea (PEG-U). To evaluate the most promising microalgae-DES, CA activity was evaluated with a specific enzymatic activity kit and through CO2 solubility assays. Preliminary results indicate that: DES is a suitable solvent medium for CA extraction from microalgal biomass, preserving its activity (specific CA activity up to 0.70 mU.mg−1); CA extraction efficiency differs between DES and microalgal species, indicating the potential for further research; from the tested DES, the ones containing PEG were favorable to maintain CA activity (CO2 solubility up to 4 g CO2.g−1 DES). This work paves the way towards a disruptive CCU approach. •Carbonic anhydrase (CA) from microalgae was extracted with deep eutectic systems (DES).•CA activity was evaluated by specific enzymatic activity kit and CO2 solubility assays.•DES is a suitable solvent for preserving CA activity.•CA extraction efficiency differs between DES and microalgal species.•DES containing PEG were more favorable to maintain CA activity.
ISSN:2212-9820
2212-9839
DOI:10.1016/j.jcou.2022.102225