Phenotypic and allelic variation for wort protein Z in Australian and Canadian barleys

• Published in: Journal of cereal science 2020-05, Vol.93, p.102935, Article 102935
• Main Authors: Luo, Hao, Zhang, Qisen, Tucek, Mark, Zhang, Xiao-Qi, Li, Chengdao
• Format: Article
• Language: English
• Subjects: Australian barley breeding; Beer foam; Malting quality; Protein Z
• ISSN: 0733-5210; 1095-9963
• DOI: 10.1016/j.jcs.2020.102935

Protein Z is a major component in beer foam. Two-dimensional electrophoresis was used to analyze wort proteins of two Australian (Buloke and Commander) and two Canadian (CDC Meredith and Bentley) varieties. The Canadian barley contained more abundant proteins from MW 40–45 kDa (pI 5 to 7). These proteins were identified as either protein Z4 or protein Z7 using liquid chromatography–mass spectrometry. Full-length gene of protein Z4 and Z7 were sequenced from Canadian and Australian barleys. Sequence differences were identified in the coding region and upstream regions of the two genes, resulting in protein sequence and expression variations. Molecular markers were designed according to the indels in the upstream regions of protein Z4 and Z7 genes. These markers were highly correlated to wort protein Z content in Canadian and Australian varieties. The Canadian barleys contained ‘high level’ genotypes for protein Z4 and Z7 while most Australian barleys had ‘low level’ genotypes for protein Z4, Z7 or both. The markers identified in this study provide a valuable tool for the selection of protein Z alleles in marker-assisted breeding. Total protein Z content was assessed using different steeping conditions, and increasing air-rest time increased protein Z content in 15 varieties. [Display omitted] •Canadian barleys had higher protein Z content in wort compared to Australian barleys.•Molecular markers were designed according to the protein Z4 and Z7 genes polymorphisms.•Protein Z4 content had high correlation with wort protein Z content.•Protein Z content was improved by increasing air-rest time.