Umbilical cord-derived mesenchymal stem cells in treatment uterine scar

• Published in: Cytotherapy (Oxford, England) England), 2020-05, Vol.22 (5), p.S66-S66
• Main Authors: Astapova, M., Ostromenskiy, V., Enukashvily, N., Kucheriavaia, I.
• Format: Article
• Language: English
• ISSN: 1465-3249; 1477-2566
• DOI: 10.1016/j.jcyt.2020.03.100

The scar on the uterus is one of the most formidable problems affecting reproductive function, that an obstetrician-gynecologist faces with. The search of the way to improve the quality of the uterine scar is extremely relevant. An experimental study aimed at comparing the tissue effects of Human MSC in the area of the wound on the uterus after surgery was carried out in rats. Methods: female albino grey rats of the Wistar line were included and operated in experiment. Model: trauma to the unchanged uterine muscle, applying with a scalpel. After suturing the uterine horns, direct intramuscular injection of MSCs (a dose of 1 × 107 cells in 1mL of 0.9% saline) (n=10, MSCs group) was injected the left-horns along uterine scar. The right horns of the uterus were not affected after suturing (n=10, control group). On the 15th day after transplantation, the rats were euthanized and serial sections of uterine tissues containing the operative region were prepared. The distribution of MSCs in the operated uterine horns of rats was evaluated by immunohistochemistry. Slides were deparaffinized and rehydrated according to a standard protocol. Before hybridization, the DNA denaturation was performed in 70% formamide at 74°C for 4 minutes. Hybridization was performed for 5 hours at 41°C with hybridization mix containing Cy3-labelled probe for human pericentromeric DNA. Image acquisition was performed using an Olympus FV3000 confocal microscope (Nikon, Japan). The cells were sectioned in z-axis with a 0.7 M interval. For large-scale 2D imaging a MatLab function built in Olympus FV 3000 software was used. Human MSC cells were detected in the left horn (where MSC had been injected) during all the 15 days of the experiment. They localized presumably in mesenchymal tissues – vessels perivascular areas, circular and longitudinal muscles. In the right horn, the cells were also detected but the number of cells was much lower. Most of hybridization signals were revealed in the nucleus. Therefore these cells can be considered as human MSC. However some of the signals were detected in the cytoplasm. These cells are most likely of the recipient origin probably the macrophages or other cells capable of phago- or endocytosis. The data obtained can expand the possibilities of pathogenetically substantiated prevention of uterine scar inferiority.