The effect of dipyridamole embedded in a drug delivery system made by electrospun nanofibers on aortic endothelial cells

An important sector of biomedical research is the generation of drug delivery systems (DDSs), which can be used alone or integrated in stents for sustained drug release. In this context, by encapsulating the platelet aggregation inhibitor dipyridamole (DIP) in polycaprolactone (PCL) we tested whethe...

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Bibliographic Details
Published in:Journal of drug delivery science and technology 2016-10, Vol.35, p.343-352
Main Authors: Repanas, Alexandros, Bader, Almke, Klett, Anne, Ngezahayo, Anaclet, Glasmacher, Birgit
Format: Article
Language:English
Subjects:
DDS
Dipyridamole
Electrospinning
Endothelial cells
Gap junction coupling
Polycaprolactone
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Summary:An important sector of biomedical research is the generation of drug delivery systems (DDSs), which can be used alone or integrated in stents for sustained drug release. In this context, by encapsulating the platelet aggregation inhibitor dipyridamole (DIP) in polycaprolactone (PCL) we tested whether we could generate a DDS with PCL by electrospinning. By characterizing the fibers' structural properties we found that fibers' diameters were affected by the concentration of DIP, which consequently had an impact on the surface area-to-volume ratio and therefore to cumulative drug release. In vitro cell studies on a bovine aortic endothelial cell line showed that PCL itself did not affect the cells according to analysis of the number of viable cells over time and metabolic activity. We found that DIP which was released from the electrospun fibers in the solution was accumulated in the cells and that it had similar effects on the cells as it had directly delivered to them. DIP inhibited the cell growth and enhanced the gap junction coupling of the endothelial cells. The results indicate that the encapsulation and the electrospinning did not affect the biological activity of DIP and show that the method can be used to generate a DDS. [Display omitted] •An electrospun DDS for sustained release of DIP was created by PCL.•The anti-proliferative effect of DIP was studied on endothelial cells.•DIP's ability to increase endothelial gap junction coupling was examined.•PCL did not have an effect on the proliferation and the gap junction coupling.•DIP inhibited the endothelial cell proliferation without cytotoxicity signs.
ISSN:1773-2247
DOI:10.1016/j.jddst.2016.08.011