In vivo SPECT-CT imaging and characterization of technetium-99m-labeled bevacizumab-loaded human serum albumin pegylated nanoparticles

• Published in: Journal of drug delivery science and technology 2021-08, Vol.64, p.101809, Article 101809
• Main Authors: Ramos-Membrive, Rocío, Erhard, Álvaro, Luis de Redín, Inés, Quincoces, Gemma, Collantes, María, Ecay, Margarita, Irache, Juan Manuel, Peñuelas, Iván
• Format: Article
• Language: English
• Subjects: Bevacizumab; Human serum albumin; Molecular imaging; Nanoparticles; SPECT/CT
• ISSN: 1773-2247
• DOI: 10.1016/j.jddst.2020.101809

The aim of this work was to study the biodistribution of bevacizumab-loaded HSA nanoparticles (NP-Ab) cross-linked with PEG35000 by SPECT/CT in vivo imaging. For this purpose, NP-Abs were prepared by a desolvation process, coated with PEG35000 and radiolabeled with technetium-99 m using a pre-tinning method ([99mTc]Tc-NP-Ab). The Ab was labeled using [99mTc][Tc(CO)3(H2O)3]+ and used to prepare nanoparticles (NP-[99mTc]Tc-Ab). Particle size was similar in both formulations. Chemical and radiochemical purity of the two nanosystems were >95%. Bevacizumab-labeling conditions were tested by in vitro stability studies. More than 87% of the radiolabeled antibody remained intact for 24 h after incubation with plasma. SPECT/CT imaging of the two nanoparticles was performed in healthy female Wistar rats. Ex vivo gamma counting of selected organs was also carried out in all animals. The results showed different clearance rates of the nanoparticle shell and the antibody, providing valuable information by the use of molecular imaging in the evaluation of drug delivery nanosystems. [Display omitted]