Nanoemulsion-based systems as a promising approach for enhancing the antitumoral activity of pequi oil (Caryocar brasilense Cambess.) in breast cancer cells

• Published in: Journal of drug delivery science and technology 2020-08, Vol.58, p.101819, Article 101819
• Main Authors: Ombredane, Alicia S., Araujo, Victor H.S., Borges, Clarissa O., Costa, Patrícia L., Landim, Marcela G., Pinheiro, Andréia C., Szlachetka, Ísis O., Benedito, Luiz E.C., Espindola, Laila S., Dias, Diego J.S., Oliveira, Daniela M., Chaker, Juliano A., da Silva, Sebastião W., de Azevedo, Ricardo B., Joanitti, Graziella A.
• Format: Article
• Language: English
• Subjects: Breast cancer; Cytotoxicity; Nanoemulsion; Pequi oil; Stability
• ISSN: 1773-2247
• DOI: 10.1016/j.jddst.2020.101819

Previous studies have reported the effects of pequi oil (Caryocar brasiliense Cambess.) in cancer prevention and treatment. However, its biomedical applications are limited due to the hydrophobic nature of the oil. In this study, we used nanotechnology strategies to design and characterize a pequi oil-based nanoemulsion and evaluate its anticancer effects. Our data show that monodisperse (PdI 0.23) pequi oil nanoemulsions (123 nm – hydrodynamic diameter; −15 mV – zeta potential) were prepared successfully using lecithin as surfactant and had physicochemical stability when exposed to stress conditions (pH and serial dilution) or long-term (200 days) storage at different temperatures. Significant dose- and time-dependent antitumoral effects against breast cancer cells (4T1) were also observed (IC50 of 299.8 and 173.7 μg/mL after 24 and 48 h respectively, p