PVA-CO-AAM and peg-co-aam hydrogels as bromelain carriers

In this paper, hydrogels were prepared by copolymerization of acrylamide (AAm) with PVA and PEG to study the incorporation and release of bromelain. The association of bromelain with polyvinyl alcohol (PVA) or polyethylene glycol (PEG) in a hydrogel for controlled release has potential for burns non...

Full description

Saved in:
Bibliographic Details
Published in:Journal of drug delivery science and technology 2021-06, Vol.63, p.102483, Article 102483
Main Authors: Coco, Julia Cedran, Tundisi, Louise Lacalendola, Santos, Érica Mendes dos, Fava, Ana Laura Masquetti, Alves, Thais Francine, Ataide, Janaína Artem, Chaud, Marco Vinícius, Mazzola, Priscila Gava
Format: Article
Language:English
Subjects:
Acrylamide
Bromelain
Controlled release
Hydrogel
PEG
PVA
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In this paper, hydrogels were prepared by copolymerization of acrylamide (AAm) with PVA and PEG to study the incorporation and release of bromelain. The association of bromelain with polyvinyl alcohol (PVA) or polyethylene glycol (PEG) in a hydrogel for controlled release has potential for burns noninvasive treatment and topical inflammatory occurrences. Total protein concentration and bromelain enzymatic activity was determined by Bradford and azocasein assay methods, respectively. Bro-PVA-co-AAm (bromelain loaded PVA hydrogels) incorporated 42.5 mg and 23.1 U, corresponding to 91.3% and 88.9%, respectively of protein content and enzymatic activity; while PEG hydrogels incorporated 41.4 mg and 16.0 U, corresponding to 88.9% and 53.8%, respectively. PVA and PEG hydrogels released less than 5% of the incorporated protein content after 24 h of study. However, release in terms of enzymatic activity reached 60% (Bro-PVA-co-AAm hydrogel) and 100% (Bro-PEG-co-AAm hydrogel). The joint analysis of hydrogels mechanical properties indicated that bromelain-loaded PVA hydrogels have more structural compatibility to biological conditions, due to a complex molecular architecture, with a lightweight, mucoadhesive, strong and soft constitution. Also, PVA-co-AAm synthesized hydrogels are able to absorb and release bromelain, indicating their potential use in a modified protein release system. [Display omitted]
ISSN:1773-2247
DOI:10.1016/j.jddst.2021.102483