Development and characterization of alginate-derived bioadhesive films incorporated with anti-infective lectins for application in the treatment of oral candidiasis

This study reports the characterization of alginate-derived bioadhesive films containing the lectins ConBr (from Canavalia brasiliensis Mart.) and MaL (from Machaerium acutifolium Vogel.). The anti-infective properties were evaluated using Tenebrio molitor larvae infected with an oral isolate of Can...

Full description

Saved in:
Bibliographic Details
Published in:Journal of drug delivery science and technology 2023-12, Vol.90, p.105114, Article 105114
Main Authors: Bazán, José Manuel Noguera, Chagas, Vitor Lopes, Silva, Raphael Guedes, Soeiro Silva, Izadora Souza, Nantes Araujo, João Guilherme, Silva, Lucas dos Santos, Batista, Karla Lilian Rodrigues, Silva, Romério Rodrigues dos Santos, Correia, Maria Tereza dos Santos, Sousa, Joicy Cortez de Sá, Monteiro, Cristina de Andrade, Tofanello, Aryane, Garcia, Wanius, Carvalho, Edilausson Moreno, Teixeira, Claudener Souza, Nascimento da Silva, Luís Cláudio
Format: Article
Language:English
Subjects:
Drug delivery
Oral candidiasis
Polysaccharide
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:This study reports the characterization of alginate-derived bioadhesive films containing the lectins ConBr (from Canavalia brasiliensis Mart.) and MaL (from Machaerium acutifolium Vogel.). The anti-infective properties were evaluated using Tenebrio molitor larvae infected with an oral isolate of Candida albicans (CA40) (1 × 104 yeast/animal). Untreated larvae showed a median survival of 1.5 days and a survival rate of 40%. Both lectins (128 μg/kg/larvae) showed prophylactic effects (survival rates: 80%–90%); however, only MaL significantly increased larval survival (70%) in the treatment assays. Bioadhesive films were developed using sodium alginate, and each lectin was incorporated at 50 μg/mL of filmogenic solution. All films (pH 6.4) showed similar micromorphology with a slightly granular appearance. The lectins-loaded films exhibited more irregular surfaces than the control. The proteins were released in similar patterns. FTIR analysis confirmed the modification of sodium alginate due to the chemical interaction with lectins. All films exhibited a two-step weight loss pattern in TGA analysis. In the ex vivo mucoadhesive assay using bovine jugal mucosa, the highest value of maximum detachment force was found for ConBr-loaded films (0.56 ± 0.04 N) (p 
ISSN:1773-2247
DOI:10.1016/j.jddst.2023.105114