Preparation of β-cyclodextrin derivatives/metamizole inclusion complex nanofibers: Characterization, drug release, and wound scratch assay

• Published in: Journal of drug delivery science and technology 2024-08, Vol.97, p.105752, Article 105752
• Main Authors: Meenatchi, Venkatasamy, Bhaskar, Rakesh, Sood, Ankur, Han, Sung Soo
• Format: Article
• Language: English
• Subjects: Cyclodextrin derivatives; Metamizole drug; MTT assay; Nanofibers; Scratch wound assay
• ISSN: 1773-2247
• DOI: 10.1016/j.jddst.2024.105752

Nanofibrous wound dressings fabricated by electrospinning have attracted significant research interest as they enable the controlled release of active substances at the wound site. The development of electrospun nanofibers with anti-inflammatory drugs, which demonstrate great potential for wound healing applications, is one of the main focuses of present research efforts. In this study, nanofibrous webs of 2-hydroxypropyl-β-cyclodextrin (HPβCD) and methyl-β-cyclodextrin (MβCD) loaded with metamizole (MMS) were fabricated by electrospinning. The prepared nanofibers were characterized by field-emission scanning electron microscopy, X-ray diffraction, Fourier-transform infrared spectroscopy, thermogravimetric/differential thermal analysis, 1H nuclear magnetic resonance spectroscopy, ultraviolet diffuse reflectance spectroscopy, and photoluminescence analysis. The interactions of HPβCD and MβCD with MMS in aqueous solutions were studied using absorption and fluorescence spectroscopy, and the 1:1 MMS/CD inclusion complexes were theoretically analyzed at the PM3 level of theory. Furthermore, the drug release, biocompatibility, and wound scratch assay of the nanofibers were evaluated. In vitro drug release analysis revealed controllable release of MMS from the nanofibers. The CD nanofibers loaded with MMS showed good biocompatibility with skin fibroblasts. An in vitro wound scratch assay indicated that the nanofibers promoted fast wound closure with a rate of 98.66 ± 3.9%. In summary, the prepared nanofibrous webs are promising candidates for wound-dressing applications. [Display omitted] •New nanofibrous webs of MMS drug were fabricated using electrospinning.•Enhanced thermal stability of MMS drug was obtained.•The controlled release of MMS from MMS/CDs-ICs-NFs was observed.•MMS/CDs-ICs-NFs exhibited excellent biocompatibility with skin fibroblasts.•MMS/CDs-ICs-NFs showed the rapid growth and migration of skin fibroblasts.