Electrospun amygdalin and Inula helenium extract-loaded polylactic acid (PLA)/Polyvinylpyrrolidone (PVP) nanofibrous patches for colon cancer treatment: Fabrication, characterization and antitumour effect results

• Published in: Journal of drug delivery science and technology 2024-09, Vol.99, p.105940, Article 105940
• Main Authors: Sulutas, Rabia Betul, Cesur, Sumeyye, Seyhan, Serap Ayaz, Alkaya, Dilek Bilgic, Sahin, Ali, Ekren, Nazmi, Gunduz, Oguzhan
• Format: Article
• Language: English
• Subjects: Amygdalin; Colon cancer; Nanofibrous patch; PLA; Plant extract; PVP
• ISSN: 1773-2247
• DOI: 10.1016/j.jddst.2024.105940

In this study, 75PLA/25PVP nanofibrous loaded with amygdalin (AMG) and Inula helenium (I.H) extract were produced by electrospinning method in order to prevent local colon cancer recurrence, and the effect of the produced nanofibrous on the HCT-116 cell line was examined in vitro. When the scanning electron microscopy (SEM) images were examined, the fiber diameter of the 75PLA/25PVP group was measured as 443.74 ± 79 nm, but the addition of I.H and AMG caused a thickening effect on the fiber diameters. Drug release results show the controlled release period of 75PLA/25PVP/I.H and 75PLA/25PVP/AMG loaded nanofibrous patches within 180 h. Slowly degrading nanofibrous remained durable for up to 25 days. When the in vitro cytotoxicity results were analyzed, all nanofibrous were found to be effective in inducing cytotoxicity against HCT-116 colon cancer cells. The most effective group is the group in which I.H extract and AMG drug are loaded onto the nanofibrous together. AMG drug and I.H plant extract showed effects on the colon cancer cell line in in vitro experiments. Considering all the results I.H and AMG loaded nanofibrous can be implanted into solid tumor cells in order to reduce the risk of local recurrence of cancer after surgery in colon cancer. [Display omitted] •The production of a nanofibrous patch consisting of PLA/PVP polymer combination loaded with amygdalin (AMG) and Inula helenium (I.H) plant extract was examined by electrospinning method to prevent colon cancer recurrence.•Inula helenium as a plant extract, amygdalin as a drug, and a plant extract-drug mixture were loaded, produced, and characterized in nanofibrous patches consisting of PLA/PVP polymer combination using the electrospun technique, both together and in a double layer, in 4 different designs in the nanofibrous patch.•A biodegradable synthetic material called PLA is appropriate for various tissue engineering applications. PVP has high hydrophilicity, thermal stability, tensile strength, solubility, bendability, and film-forming properties.•The therapeutic effect of a layered nanofibrous patch cover produced by the electrospinning method and combinations of two active ingredients on colon cancer cells.