Oncostatin M sensitizes keratinocytes to UVB-induced inflammation via GSDME-mediated pyroptosis

• Published in: Journal of dermatological science 2021-11, Vol.104 (2), p.95-103
• Main Authors: Liu, Jun, Zhong, Yadan, Liu, Huiting, Yang, Huan, Lu, Ping, Shi, Yanqiang, Wang, Xuan, Zheng, Wen, Yu, Xiaoling, Xu, Yingping, Yang, Bin
• Format: Article
• Language: English
• Subjects: Animals; Epidermis - metabolism; Gene Knockout Techniques; HaCaT Cells; Humans; Interleukin-1beta - genetics; Interleukin-1beta - metabolism; Keratinocytes - radiation effects; Mice; Mice, Knockout; NLR Family, Pyrin Domain-Containing 3 Protein - genetics; NLR Family, Pyrin Domain-Containing 3 Protein - metabolism; Oncostatin M - pharmacology; Oncostatin M Receptor beta Subunit - genetics; Pore Forming Cytotoxic Proteins - genetics; Pore Forming Cytotoxic Proteins - metabolism; Pyroptosis - drug effects; Pyroptosis - genetics; Pyroptosis - radiation effects; RNA, Small Interfering; Ultraviolet Rays; Up-Regulation
• ISSN: 0923-1811; 1873-569X
• DOI: 10.1016/j.jdermsci.2021.09.004

•OSM is a priming signal that enhances KC-mediated inflammation via pyroptosis induction.•OSM upregulates inflammasome/pyroptosis components in KC.•GSDME mediates OSM-induced KC pyroptosis.•OSM sensitizes KC to UVB-induced pyroptosis in human epidermis. [Display omitted] Oncostatin M (OSM), an interleukin-6 (IL-6) family proinflammatory cytokine, plays a critical role in inflammatory skin diseases, but its mechanism of action is not well understood. To demonstrate the mechanism of OSM induced pyropotosis in normal human epidermal keratinocytes (NHEKs) and immortalized human keratinocytes (HaCaT cells). NHEKs and HaCaT cells were treated with OSM. Knockout of OSM receptor (OSMR) with CRISPR/Cas9 system, knockdown of GSDME with small interfering RNA and primary keratinocytes from Osmr−/− and Gsdme−/− mice were used to study the effect of OSMR and GSDME. After treatment of OSM, NHEKs and HaCaT cells were irradiated with UVB. The mRNA was analyzed by quantitative real-time polymerase chain reaction (qRT-PCR) and RNA sequencing, protein level was detected by Western Blotting, Elisa and immunofluorescence. Cell death was examined by lactate dehydrogenase (LDH) releasing. Here we found that OSM induced pyropotosis in NHEKs and HaCaT cells, but knockout of OSMR abolished pyropotosis. RNA sequencing revealed an upregulation of several key genes involved in NLRP3 inflammasome activation following OSM treatment, among which NLRP3, GSDME, and IL-1β were confirmed by qRT-PCR and Western Blotting. Knockdown of GSDME alleviated OSM-induced pyropotosis. Pretreatment of OSM boosted UVB-induced pyroptosis and inflammation in NHEKs and HaCaT cells, and this priming function was lost in keratinocytes of Osmr−/− and Gsdme−/− mice. Similar results were obtained in a 3-dimensional culture of human epidermis. OSM functions as a priming cytokine to enhance UVB-induced inflammation in keratinocytes, providing insight into the pathogenesis of inflammatory skin diseases.