Enzymeless creatinine estimation using poly(3,4-ethylenedioxythiophene) -β-cyclodextrin

• Published in: Journal of electroanalytical chemistry (Lausanne, Switzerland) Switzerland), 2011, Vol.661 (2), p.303-308
• Main Authors: Naresh Kumar, Thangavel, Ananthi, Arjunan, Mathiyarasu, Jayaraman, Joseph, James, Lakshminarasimha Phani, Kanala, Yegnaraman, Venkatraman
• Format: Article
• Language: English
• Subjects: ammonium compounds; Analytical biochemistry: general aspects, technics, instrumentation; Analytical, structural and metabolic biochemistry; aqueous solutions; ascorbic acid; beta-cyclodextrin; Biological and medical sciences; biosensors; carbon; Chemistry; Creatinine; Cyclodextrin; detection limit; Electrochemistry; electrodes; Electrodes: preparations and properties; Enzymeless; Exact sciences and technology; Fundamental and applied biological sciences. Psychology; General and physical chemistry; glucose; Impedance; lithium; Other electrodes; PEDOT; polymers; Potentiometry; scanning electron microscopy; shelf life; ultraviolet-visible spectroscopy; urea; uric acid
• ISSN: 1572-6657; 1873-2569
• DOI: 10.1016/j.jelechem.2011.08.001

► Possibility of fabricating an enzyme-less potentiometric creatinine sensor using a simple electrode modification of PEDOT ► Non-covalent interactions of creatinine with βCD are exploited for molecular recognition of creatinine ► The potentiometric sensor yields reasonably stable response in pH 7.0 buffer solutions in a range of 10 −5–10 −1 M. A novel enzymeless biosensor has been constructed for the quantitative estimation of creatinine using β-cyclodextrin (βCD) incorporated poly-3,4-ethylenedioxythiophene (PEDOT) modified glassy carbon electrode (GCE). The PEDOT film with and without βCD was deposited on a GCE by electropolymerization of EDOT from an aqueous solution containing lithium perchlorate and βCD. Polymer films were characterized by UV–visible spectroscopy, cyclic voltammetry and scanning electron microscopy (SEM). The selective interactions of the βCD-incorporated PEDOT film with creatinine in neutral Tris buffer solutions were elucidated using electrochemical impedance analysis. Complex formation between βCD and creatinine was inferred from regular shifts in the electrode potential versus creatinine concentration. In the potentiometric evaluation, the biosensor electrode exhibited a fast response time (60 s), linear shift in the potential over a concentration range of 10 −4–10 −1 M with a lower detection limit of 5 × 10 −5 M and good stability, retaining nearly 95% of the initial response after 1 month of shelf life. The results of the studies on the interferences from glucose, ascorbic acid, uric acid, urea and ammonium ions are also presented.