Anodized screen-printed electrode modified with poly(5-amino-4H-1,2,4-triazole-3-thiol) film for ultrasensitive detection of Hg2+ in fish samples

• Published in: Journal of electroanalytical chemistry (Lausanne, Switzerland) Switzerland), 2023-01, Vol.929, p.117121, Article 117121
• Main Authors: Manikandan, Ramalingam, Pugal Mani, S., Sangeetha Selvan, Kumar, Yoon, Jang-Hee, Chang, Seung-Cheol
• Format: Article
• Language: English
• Subjects: Chemically modified electrodes; Electrochemical detection; Electrochemical polymerization; Fish muscles; Hg2; ICP-MS
• ISSN: 1572-6657; 1873-2569
• DOI: 10.1016/j.jelechem.2022.117121

[Display omitted] •A facile single-step method was used to prepare PATT film on ASPE surface.•PATT film was coated on the surface via stable amide linkage.•The prepared PATT@ASPE has a large surface area with excellent electrochemical properties.•PATT@ASPE was used for detecting Hg2+ in different fish muscle samples. Anodized screen-printed electrode (ASPE) was modified by electrochemical polymerization of 5-amino-4H-1,2,4-triazole-3-thiol (ATT) to form a poly-ATT (PATT) film for the selective and sensitive detection of Hg2+ ions by Anodic Stripping Voltammetry (ASV). The porous PATT film was uniformly coated on the surface through disulfide (SS) linkage, free NH, and amide bonds for binding preconcentrated Hg2+ ions. The prepared PATT@ASPE was characterized by cyclic voltammetry, Field-Emission Scanning Electron Microscopy (FE-SEM), Transmission Electron Microscopy (TEM) with Energy Dispersive X-ray spectroscopy (EDS) and Attenuated Total Reflection Infrared Spectroscopy (ATR-IR). Under optimized experimental conditions (0.1 M KCl as supporting electrolyte and preconcentration at –0.1 V for 120 s), PATT@ASPE was successfully used for detecting Hg2+. A linear relationship between the anodic peak current and [Hg2+] was achieved in the concentration range of 0.03 to 245 nM, with a limit of detection (S/N = 3) of 0.005 nM and a calculated sensitivity of 15.0085 µAnM−1L-1 cm−2. Further, selective detection of Hg2+ was demonstrated in the presence of interfering species such as other heavy metal ions (Pb2+, Cd2+, Cu2+, etc.), organic molecules, other cations, and various anions. The electroanalytical performance of PATT@ASPE exhibited outstanding long-term stability and reproducibility. Finally, PATT@ASPE was successfully applied to measure Hg2+ in fish muscle samples, and the results were validated using inductively coupled plasma-mass spectrometry.