Electrochemical behavior of catalase at electrified liquid-organogel interface

• Published in: Journal of electroanalytical chemistry (Lausanne, Switzerland) Switzerland), 2024-03, Vol.956, p.118093, Article 118093
• Main Authors: Sudalaimani, S., Santhosh, B., Esokkiya, A., Kumar, K. Sanjeev, Sivakumar, C., Suresh, C., Giribabu, K.
• Format: Article
• Language: English
• Subjects: Antioxidant; Catalase; Electrified soft interface; Ion transfer voltammetry; ITIES; Sensors
• ISSN: 1572-6657; 1873-2569
• DOI: 10.1016/j.jelechem.2024.118093

[Display omitted] •Catalase studied at electrified soft interfaces using plastic micropipette tip.•Adsorbed catalase at interface shows increase in current upon addition of H2O2.•The proposed method shows a good analytical performance in artificial serum. In this work, we have studied the electrochemical behavior of catalase at the electrified soft interface using ion transfer voltammetry. The effect of catalase concentration, influence of electrolytes at both the aqueous phase and organic phase on the interfacial behavior were investigated. From the ion transfer voltammogram, electroanalytical parameters such as limit of detection, sensitivity have been calculated with respect to catalase at different aqueous and organic environments. In order to move the optimized method closer to practical use, we designed a plastic micropipette tip with an aperture facilitating a gentle connection. Fabricated plastic micropipette tip supported electrochemical set-up has been validated using electrochemical (ion transfer voltammetry) and physical (scanning electron microscopy) characterizations. Electroanalytical ability of catalase in acidic aqueous phase at the liquid-organogel interface exhibited a linear range of 1–20 μM with a detection limit of 0.46 μM and 0.42 μM and a sensitivity of 4.15 nA μM−1 and 4.97 nA μM−1 with respect to forward and backward scan respectively. The fabricated plastic micropipette tip supported organogel interface finds application in the area of label-free sensing of biomacromolecules (catalase). Moreover, the adsorbed catalase at the interface has been confirmed through H2O2 as the probe. It will open-up an affordable sensing platform for H2O2 detection as well.