Arbuscular mycorrhizal fungi on growth, nutrient status, and total antioxidant activity of Melilotus albus during phytoremediation of a diesel-contaminated substrate

• Published in: Journal of environmental management 2012-03, Vol.95, p.S319-S324
• Main Authors: Hernández-Ortega, Herminia Alejandra, Alarcón, Alejandro, Ferrera-Cerrato, Ronald, Zavaleta-Mancera, Hilda Araceli, López-Delgado, Humberto Antonio, Mendoza-López, Ma. Remedios
• Format: Article
• Language: English
• Subjects: Agricultural Inoculants; alkaline phosphatase; AMF; antioxidant activity; Antioxidants; Antioxidants - metabolism; Biodegradation, Environmental; Biomass; Fungal alkaline phosphatase; Gasoline; Glomus; hydrocarbons; Hydrocarbons - analysis; leaf area; leaves; Melilotus - growth & development; Melilotus - metabolism; Melilotus - microbiology; Melilotus albus; Mycorrhizae - metabolism; mycorrhizal fungi; nitrate reductase; Nitrate Reductase - metabolism; nutrient content; nutritional status; petroleum; Petroleum hydrocarbons; Petroleum Pollution; Phenols - analysis; Phenols - metabolism; Phytoremediation; Phytotoxicity; plant growth; Plant Leaves - growth & development; Plant Shoots - growth & development; planting; roots; sand; seedlings; shoots; Soil Pollutants; toxicity
• ISSN: 0301-4797; 1095-8630
• DOI: 10.1016/j.jenvman.2011.02.015

This research evaluated the effects of arbuscular mycorrhizal fungi (AMF) on growth, nutritional status, total antioxidant activity (AOX), total soluble phenolics content (TPC), and total nitrate reductase activity (NRA) of leaves and roots of Melilotus albus Medik planted in diesel-contaminated sand (7500 mg kg−1). Seedlings of Melilotus either Non inoculated (Non-AMF) or pre-inoculated plants (AMF) with the AMF-inoculum Glomus Zac-19 were transplanted to non-contaminated or contaminated sand. After 60 days, diesel significantly reduced plant growth. AMF- plants had no significant greater (64% and 89%, respectively) shoot and leaf dry weight than Non-AMF plants, but AMF plants had lower specific leaf area. AMF-plants had significantly greater content of microelements than non-AMF plants. Regardless diesel contamination, the total AOX and TPC were significantly higher in leaves when compared to roots; in contrast, NRA was higher in roots than leaves. Diesel increased total AOX of leaves, but AMF-plants had significantly lower AOX than non-AMF plants. In contrast, roots of AMF-plants had significantly higher AOX but lower NRA than non-AMF plants. AMF-colonization in roots detected via the fungal alkaline phosphatase activity was significantly reduced by the presence of diesel. AMF-inoculation alleviated diesel toxicity on M. albus by enhancing plant biomass, nutrient content, and AOX activity. In addition, AMF-plants significantly contributed in higher degradation of total petroleum hydrocarbons when compared to non-AMF-plants.