Anti-venom potential of butanolic extract of Eclipta prostrata against Malayan pit viper venom

• Published in: Journal of ethnopharmacology 2004-02, Vol.90 (2), p.347-352
• Main Authors: Pithayanukul, Pimolpan, Laovachirasuwan, Sasitorn, Bavovada, Rapepol, Pakmanee, Narumol, Suttisri, Rutt
• Format: Article
• Language: English
• Subjects: Animal poisons toxicology. Antivenoms; Animals; Anti-snake venom; Antivenins - chemistry; Antivenins - pharmacology; Antivenins - therapeutic use; Biological and medical sciences; Butanols - chemistry; Butanols - pharmacology; Butanols - therapeutic use; Calloselasma rhodostoma; Crotalid Venoms - antagonists & inhibitors; Crotalid Venoms - toxicity; Demethylwedelolactone; Dose-Response Relationship, Drug; Eclipta; Eclipta prostrata; Female; General pharmacology; Hemorrhage - chemically induced; Hemorrhage - drug therapy; Malayan pit viper; Male; Medical sciences; Mice; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; Plant Components, Aerial; Plant Extracts - chemistry; Plant Extracts - pharmacology; Plant Extracts - therapeutic use; Toxicology; Viperidae
• ISSN: 0378-8741; 1872-7573
• DOI: 10.1016/j.jep.2003.10.014

The butanolic and purified butanolic extracts (PBEs) of Eclipta prostrata were evaluated for their anti-venom potential. Inhibition of lethal, hemorrhagic, proteolytic, and phospholipase A 2 activities of Calloselasma rhodostoma (Malayan pit viper (MPV)) venom by these extracts were determined. Demethylwedelolactone was identified as their major constituent. The butanolic extract, at 2.5 mg per mouse, was able to completely neutralize the lethal activity of 2LD 50 of MPV venom, but increasing the dose diminished the effect. The PBE, at 1.5–4.5 mg per mouse, was able to neutralize the lethality of the venom at around 50–58%. Both extracts partially inhibited the hemorrhagic activity but displayed very low anti-phospholipase A 2 activity and did not inhibit proteolytic activity of MPV venom.