Hepatoprotective effects of Coptidis rhizoma aqueous extract on carbon tetrachloride-induced acute liver hepatotoxicity in rats

• Published in: Journal of ethnopharmacology 2009-07, Vol.124 (1), p.130-136
• Main Authors: Ye, Xingshen, Feng, Yibin, Tong, Yao, Ng, Kwan-Ming, Tsao, SaiWah, Lau, George K.K., Sze, Chowing, Zhang, Yanbo, Tang, Jun, Shen, Jiangang, Kobayashi, Seiichi
• Format: Article
• Language: English
• Subjects: Alanine aminotransferase; alanine transaminase; Alanine Transaminase - blood; animal models; Animals; antioxidant activity; Aspartate aminotransferase; Aspartate Aminotransferases - blood; aspartate transaminase; Berberine - pharmacology; Berberine - therapeutic use; Biological and medical sciences; body weight; Carbon Tetrachloride; Chemical and Drug Induced Liver Injury - prevention & control; Coptidis rhizoma aqueous extract; Coptis; Coptis - chemistry; Coptis chinensis; dosage; dose response; Drugs, Chinese Herbal - pharmacology; Drugs, Chinese Herbal - therapeutic use; enzyme inhibitors; General pharmacology; hepatoprotective effect; hepatotoxicity; histopathology; Liver - drug effects; Liver - pathology; Liver histopathology; Male; mechanism of action; Medical sciences; medicinal plants; medicinal properties; oral administration; Oriental traditional medicine; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; Phytotherapy; plant extracts; Protective Agents - pharmacology; Protective Agents - therapeutic use; Rats; Rats, Sprague-Dawley; Rhizome; rhizomes; Superoxide dismutase; Superoxide Dismutase - metabolism
• ISSN: 0378-8741; 1872-7573
• DOI: 10.1016/j.jep.2009.04.003

Coptidis rhizoma (CR, Chinese name is Huanglian) has been used in treating infectious and inflammatory diseases for two thousand years in Traditional Chinese Medicine (TCM). Its related pharmacological basis for the therapeutics has been studied intensively, but CR can also be used for vomiting of “dampness-heat type or acid regurgitation” due to “liver-fire attacking stomach” in TCM, whose symptoms seem to link the hepatic and biliary disorders, yet details in the therapies of liver diseases and underlying mechanism(s) remain unclear. To clarify this ethnopharmacological relevance, hepatoprotective effect of Coptidis rhizoma aqueous extract (CRAE) and its possible mechanism were studied in rats intoxicated with carbon tetrachloride (CCl4) in the present study. Sprague–Dawley (SD) rats aged 7 weeks old were intraperitoneally injected with CCl4 at a dose of 1.0ml/kg as a 50% olive oil solution. The rats were orally given the CRAE at doses of 400, 600, 800mg/kg and 120mg/kg berberine body weight (BW) after 6h of CCl4 treatment. At 24h after CCl4 injection, samples of blood and liver were collected and then biochemical parameters and histological studies were carried out. The results showed that CRAE and berberine inhibited significantly the activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) and increased the activity of superoxide dismutase (SOD). Observation on the hepatoprotective effect of berberine was consistent to that of CRAE. The study is the first time to demonstrate that CRAE has hepatoprotective effect on acute liver injuries induced by CCl4, and the results suggest that the effect of CRAE against CCl4-induced liver damage is related to antioxidant property.