Inhibitory effects of Geijigajakyak-Tang on trinitrobenzene sulfonic acid-induced colitis

• Published in: Journal of ethnopharmacology 2009-11, Vol.126 (2), p.244-251
• Main Authors: Lee, Ji Yun, Kang, Hyo Sook, Park, Byoung Eon, Moon, Hyo Jin, Sim, Sang Soo, Kim, Chang Jong
• Format: Article
• Language: English
• Subjects: Acrolein - analogs & derivatives; Acrolein - pharmacology; Acrolein - therapeutic use; animal models; Animals; anti-inflammatory activity; Anti-Inflammatory Agents - pharmacology; Anti-Inflammatory Agents - therapeutic use; Antioxidants - pharmacology; Antioxidants - therapeutic use; Benzoates - pharmacology; Benzoates - therapeutic use; Biological and medical sciences; Bridged-Ring Compounds - pharmacology; Bridged-Ring Compounds - therapeutic use; chemotaxis; Chemotaxis - drug effects; colitis; Colitis - chemically induced; Colitis - drug therapy; colon; Cyclohexanols - pharmacology; Cyclohexanols - therapeutic use; enzyme activity; Female; Geijigajakyak-Tang; General pharmacology; Glucosides - pharmacology; Glucosides - therapeutic use; Glycyrrhizic Acid - pharmacology; Glycyrrhizic Acid - therapeutic use; herbal medicines; ileum; Ileum - drug effects; inhibitory concentration 50; Lipid Peroxidation - drug effects; Magnoliopsida - chemistry; Malondialdehyde; Malondialdehyde - metabolism; mechanism of action; Medical sciences; medicinal plants; medicinal properties; Mice; Mice, Inbred BALB C; Models, Animal; Monoterpenes - pharmacology; Monoterpenes - therapeutic use; Muscle contraction; Muscle Contraction - drug effects; Muscle, Smooth - drug effects; Neutrophil chemotaxis; neutrophils; Neutrophils - drug effects; Oriental traditional medicine; Parasympatholytics - pharmacology; Parasympatholytics - therapeutic use; Peroxidase - metabolism; peroxidases; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; Phytotherapy; Plant Extracts - chemistry; Plant Extracts - pharmacology; Plant Extracts - therapeutic use; protective effect; rats; Saponins - pharmacology; Saponins - therapeutic use; smooth muscle; TNBS-induced colitis; Trinitrobenzenesulfonic Acid; Triterpenes - pharmacology; Triterpenes - therapeutic use
• ISSN: 0378-8741; 1872-7573
• DOI: 10.1016/j.jep.2009.08.035

Water extract of Geijigajakyak-Tang (GJT) consisting of five crude drugs [dried root of P. lactiflora Peony (Paeoniaceae), dried trunk bark of C. cassia Blume (Lauraceae), seed of Z. jujube var. inermis Mill (Rhamnaceae), fresh root of Z. officinale Rocoe (Zingiberaceae) and dried trunk bark of G. uralensis Fish (Leguminosae)] is a folk medicine used for the treatment of chronic colitis. This study was designed to further elucidate the effect of GJT on 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced colitis in rats. GJT orally given to mice before and after TNBS intoxication, and their clinical and morphological changes, myeloperoxidase (MPO) activity and malondialdehyde (MDA) levels in colon tissues, were evaluated on Day 8 post-TNBS. Furthermore, the effect of six major constituents of individual herbs on ileum smooth muscle contraction and neutrophil chemotaxis was studied. GJT had a significant anti-inflammatory effect based on clinical and morphologic changes, MPO activity and MDA levels in colon tissues as compared with sham control. GJT and 5 major active constituents of individual herbs, paeoniflorin, cinnamaldehyde, jujuboside A, jujubogenin, and diammonium glycyrhhizinate significantly inhibited neutrophil chemotaxis. GJT significantly inhibited muscle contraction (IC 50; 2.10 ± 0.11 mg/ml), and 1,8-cineol has the most spasmolytic activity (IC 50; 0.10 ± 0.03 mg/ml). GJT has significant anti-inflammatory effects on TNBS-induced colitis via inhibitions of smooth muscle contraction and neutrophil chemotaxis.