Effect of Emblica officinalis (fruit) against UVB-induced photo-aging in human skin fibroblasts

• Published in: Journal of ethnopharmacology 2010-10, Vol.132 (1), p.109-114
• Main Authors: Adil, Mushtaq D., Kaiser, Peerzada, Satti, Naresh K., Zargar, Afzal M., Vishwakarma, Ram A., Tasduq, Sheikh A.
• Format: Article
• Language: English
• Subjects: Biological and medical sciences; cell cycle; Cell Cycle - drug effects; Cell Cycle - radiation effects; Cell Line; cell proliferation; Cell Survival - drug effects; Cell Survival - radiation effects; Chromatography, High Pressure Liquid; collagen; Collagen Type I - metabolism; Cytoprotection - drug effects; Cytoprotection - radiation effects; Emblica officinalis; enzyme activity; enzyme inhibition; fibroblasts; Fibroblasts - drug effects; Fibroblasts - metabolism; Fibroblasts - radiation effects; free radical scavengers; Fruit - chemistry; fruits (plant anatomy); General pharmacology; Humans; hyaluronic acid; hyaluronoglucosaminidase; Hyaluronoglucosaminidase - antagonists & inhibitors; Matrix Metalloproteinase 1 - metabolism; Medical sciences; medicinal plants; medicinal properties; Medicine, Ayurvedic; metalloproteinases; mitochondria; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; Photo-aging; Phyllanthus emblica; Phyllanthus emblica - chemistry; Plant Extracts - isolation & purification; Plant Extracts - pharmacology; Procollagen - metabolism; quantitative analysis; reactive oxygen species; senescence; skin; Skin - cytology; Skin - drug effects; Skin - metabolism; Skin - radiation effects; Skin Aging - drug effects; Skin Aging - radiation effects; Skin fibroblast; ultraviolet radiation; Ultraviolet Rays - adverse effects; UVB
• ISSN: 0378-8741; 1872-7573
• DOI: 10.1016/j.jep.2010.07.047

Emblica officinalis fruit (EO), commonly known as Amla is a reputed traditional medicine and functional food used in Indian subcontinent. It has long been used in Indian folk medicine to treat liver diseases, stomach ulcers, inflammatory diseases, metabolic disorders, geriatric complaints, skin disorders and beauty care. Recently, it has been shown to promote pro-collagen content and inhibit matrix metalloproteinase levels in skin fibroblast. The aim of the present study was to investigate the efficacy of EO to inhibit UVB-induced photo-aging in human skin fibroblasts. Mitochondrial activity of human skin fibroblasts was measured by MTT-assay. Quantifications of pro-collagen 1 and matrix metalloproteinase 1 (MMP-1) release were performed by immunoassay techniques. Hyaluronidase inhibition assay was studied in vitro using bovine testicular hyaluronidase and human umbilical cord hyaluronic acid. Cell cycle analysis was performed by flowcytometry using propidium iodide. EO stimulated, the otherwise UVB inhibited cellular proliferation and protected pro-collagen 1 against UVB-induced depletion via inhibition of UVB-induced MMP-1 in skin fibroblasts (10–40 μg/mL, p > 0.001). EO exhibited inhibitory activity of hyaluronidase (10–40 μg/mL, p > 0.001). Treatment with EO also prevented UVB disturbed cell cycle to normal phase. The results of the present study suggests that EO effectively inhibits UVB-induced photo-aging in human skin fibroblast via its strong ROS scavenging ability and its therapeutic and cosmetic applications remain to be explored.