In vivo antimalarial activity, toxicity and phytochemical screening of selected antimalarial plants

• Published in: Journal of ethnopharmacology 2013-03, Vol.146 (2), p.557-561
• Main Authors: Musila, M.F., Dossaji, S.F., Nguta, J.M., Lukhoba, C.W., Munyao, J.M.
• Format: Article
• Language: English
• Subjects: Adansonia; Adansonia digitata; alkaloids; animal models; Animals; Antimalarial activity; Antimalarials - pharmacology; Artemia; Artemia - drug effects; Asteraceae; bark; Canthium; drugs; flavonoids; indigenous peoples; Kenya; Launaea; Lethal Dose 50; malaria; Medicinal plants; Medicine, African Traditional; mice; Parasitic Sensitivity Tests; Phytochemical screening; Plant Extracts - pharmacology; Plasmodium berghei; Plasmodium berghei - drug effects; Rubiaceae; screening; sesquiterpenoid lactones; Toxicity; Toxicity Tests, Acute; traditional medicine; Zanthoxylum
• ISSN: 0378-8741; 1872-7573
• DOI: 10.1016/j.jep.2013.01.023

Malaria continues to kill over a million people each year and in many populations affected by malaria, conventional drugs are often unaffordable or inaccessible. Historically, plants have been a prominent source of antimalarial drugs. Those plants currently used by indigenous people to treat malaria should be documented and investigated as potential sources of new antimalarial drugs. To investigate in vivo antimalarial activity, toxicity and carry out phytochemical screening of selected plants which have been used in traditional medicine for the treatment of malaria. Organic and water extracts of four medicinal plants used for the treatment of malaria in traditional health systems of Msambweni people in Kenya were tested for antimalarial activity against Plasmodium berghei and brine shrimp lethality. They were also screened for their major phytochemical constituents. Aqueous extract of the stem bark of Adansonia digitata exhibited highest chemosuppression of parasitaemia, >60% in a murine model of Plasmodium berghei infected mice. Aqueous and organic extracts of Launaea cornuta and Zanthoxylum chalybeum were toxic to the brine shrimp (LD501000μg/ml). Phytochemical screening revealed the presence of alkaloids and flavonoids in all the crude extracts of the selected plant species studied. Sesquiterpene lactones and saponis were present in organic extracts but absent in the aqueous extracts of Adansonia digitata, Canthium glaucum, Launaea cornuta and Zanthoxylum chalybeum. The results showed that the crude extracts of Adansonia digitata and Canthium glaucum demonstrated promising antimalarial activity and there is potential for isolation of lead compounds from their extracts. Key: A=Aqueous extract, B=CHCl3: MeOH (1:1) extract. [Display omitted]