Assessment of the genotoxic and mutagenic properties of Himatanthus articulatus bark extracts used as phytotherapeutic drug in the Amazon

• Published in: Journal of ethnopharmacology 2013-05, Vol.147 (2), p.474-480
• Main Authors: Rebouças, Sebastião de O., da Silva, Juliana, Bertoni, Ricardo S., Decker, Natalia, Santos, Marcela Silva dos, Rossatto, Raíssa R., Corrêa, Dione S., Ferraz, Alexandre B.F.
• Format: Article
• Language: English
• Subjects: Animals; Apocynaceae; bark; blood; Bone Marrow - drug effects; Cells, Cultured; Comet Assay; DNA; drugs; Erythrocytes - drug effects; Ethanol - chemistry; Female; Flavonoids - analysis; Flavonoids - pharmacology; Furans - isolation & purification; Furans - pharmacology; gender differences; Genotoxicity; high performance liquid chromatography; Himatanthus articulatus; hydrogen peroxide; indigenous species; Iridoid; iridoids; malaria; Male; Medicine, Traditional; Mice; Micronucleus Tests; Mutagenicity; neoplasms; phenolic compounds; Phenols - analysis; Phenols - pharmacology; phytochemicals; Plant Bark - chemistry; Plant Extracts - chemistry; Plant Extracts - pharmacology; Plumieride; Solvents - chemistry; South America; Spiro Compounds - isolation & purification; Spiro Compounds - pharmacology; Tannins - analysis; Tannins - pharmacology; Water - chemistry
• ISSN: 0378-8741; 1872-7573
• DOI: 10.1016/j.jep.2013.03.041

Himatanthus articulatus (Apocynaceae) is a plant native to the Amazon, popularly used to treat external ulcers, tumors, inflammations, cancer, syphilis and malaria. To investigate the in vivo genotoxic and mutagenic potential of this plant, using the comet assay and the micronucleus test. Female and male adult mice were treated with 500mg/kg, 1000mg/kg or 2000mg/kg of Himatanthus articulatus aqueous or ethanolic bark extracts by gavage for two consecutive days. In addition, blood slides were exposed to hydrogen peroxide (ex vivo) to evaluate the anticlastogenic effect using the comet assay. The HPLC analyses indicated plumieride as the main constituent of both extracts from Himatanthus articulatus barks. No differences between genders were observed. Micronuclei were observed only in the group treated with the highest dose of both extracts. Conversely, lower doses of these extracts showed protective effects to DNA against damage induced by hydrogen peroxide, indicating an important antigenotoxic effect. The toxicological evaluation indicated that the extracts are non-genotoxic and reduce the clastogenic damage induced by hydrogen peroxide. In part, this result can be atributted to the phytochemical profile of Himatanthus articulatus, which presents iridoids and phenolic compounds. Using the comet assay, the Himatanthus articulatus extracts showed antigenotoxic effect protecting blood cells against the damages induced by H2O2 [Display omitted]