Antidiabetic effect of Cordia morelosana, chemical and pharmacological studies

• Published in: Journal of ethnopharmacology 2020-04, Vol.251, p.112543, Article 112543
• Main Authors: Giles-Rivas, Diana, Estrada-Soto, Samuel, Aguilar-Guadarrama, A. Berenice, Almanza-Pérez, Julio, García-Jiménez, Sara, Colín-Lozano, Blanca, Navarrete-Vázquez, Gabriel, Villalobos-Molina, Rafael
• Format: Article
• Language: English
• Subjects: 3T3-L1 Cells; alpha-Glucosidases - metabolism; Animals; Cordia; Cordia morelosana standley; Diabetes; Diabetes Mellitus, Experimental - drug therapy; Diabetes Mellitus, Type 2 - drug therapy; Fatty Acid-Binding Proteins - genetics; Glucose Transporter Type 4 - genetics; Hypoglycemic Agents - chemistry; Hypoglycemic Agents - pharmacology; Hypoglycemic Agents - therapeutic use; Hypoglycemic Agents - toxicity; Male; Methyl rosmarinate; Mice; Phytochemicals - analysis; Phytochemicals - pharmacology; Phytochemicals - therapeutic use; Phytochemicals - toxicity; Plant Extracts - chemistry; Plant Extracts - pharmacology; Plant Extracts - therapeutic use; Plant Extracts - toxicity; PPAR alpha - genetics; PPAR alpha - metabolism; PPAR gamma - genetics; PPAR gamma - metabolism; PPARα; PPARγ; Rats, Sprague-Dawley; Rats, Wistar; Toxicity Tests, Acute; Toxicity Tests, Subchronic; α-Glucosidases
• ISSN: 0378-8741; 1872-7573
• DOI: 10.1016/j.jep.2020.112543

Standley (Boraginaceae) is commonly used in folk medicine for the treatment of diarrhoea, kidney inflammation, diabetes, lung pain, bronchitis, asthma, hoarseness, cough and fever. Current work was conducted to develop a bio-guided isolation of antidiabetic compounds from ethanolic extract of Cordia morelosana (EECm). The phytochemical bio-guided study was conducted by successive chromatographic techniques, and isolated compounds were characterized by 1D and 2D-NMR experiments. The in vivo antihyperglycemic and antidiabetic activities of EECm (100 mg/kg), and methyl rosmarinate (MR, 50 mg/kg) were determined on normoglycemic and diabetic murine models. Additionally, the in vitro activity was conducted to determine α-glucosidase inhibitory effect, and PPARs, GLUT4 and FATP expression on 3T3-L1 cells by RT-PCR. Acute and sub-chronic toxicological studies for EECm were conducted on rats, following the OECD guidelines (No. 420 and 407). EECm promotes significant α-glucosidase inhibition (55.6%) at 1 mg/kg respect to the control. Also, EECm (100 mg/kg) showed significant antihyperglycemic effect on oral glucose tolerance test (OGTT), and in non-insulin dependent type 2 diabetes (NIDD) model, had antidiabetic activity (p