Un-riped fruit pods of Prosopis cineraria (L.) Druce ameliorates Cisplatin therapy-induced partial testicular atrophy in male Wistar rats

• Published in: Journal of ethnopharmacology 2020-10, Vol.261, p.113070, Article 113070
• Main Authors: Ramalingam S, Logeshwaran, Vellapandian, Chitra, R Vetrivelan, Venkataramanan, Sukumaran, Evelyn Sharon
• Format: Article
• Language: English
• Subjects: Animals; Atrophy; Cisplatin; Disease Models, Animal; Endocrine system; Female; Fertility; Fertility - drug effects; Flavonoids; Fruit - chemistry; Hormones - blood; Infertility, Male - chemically induced; Infertility, Male - metabolism; Infertility, Male - physiopathology; Infertility, Male - prevention & control; Litter Size - drug effects; Male; Oxidative Stress - drug effects; Plant Extracts - isolation & purification; Plant Extracts - pharmacology; Pregnancy; Prosopis - chemistry; Rats, Wistar; Reproductive pharmacology; Sperm Count; Sperm Motility - drug effects; Spermatozoa - drug effects; Spermatozoa - metabolism; Spermatozoa - pathology; Steroids; Testicular Diseases - chemically induced; Testicular Diseases - metabolism; Testicular Diseases - pathology; Testicular Diseases - prevention & control; Testis - drug effects; Testis - pathology
• ISSN: 0378-8741; 1872-7573
• DOI: 10.1016/j.jep.2020.113070

Prosopis cineraria (L.) Druce is a plant that is widely found in dry parts of India. The unripe fruit pod has a very specific traditional claim of treating male infertility and increasing sperm volume and count. The present work was endeavored to investigate the long-standing traditional claim of P. cineraria on meliorating male fertility. The study focussed on cancer therapy-induced male infertility and curative effect of the extract with an appraisal on any possible revitalizing effects on sperm count, morphology, motility, and viability combined with hormonal and histopathological investigations. Male Wistar rats were used for the study. Two different doses of 400 mg/kg/d and 800 mg/kg/d (both p.o.) of the Hydroalcoholic extract were chosen as test dose while Clomiphene (25 mg/kg/d; p.o.) treatment served as standard treatment. Animals were initially injected with cisplatin (1 mg/kg/d; i.p.) for 15 days and the drug treatment was begun at the 16th day and continued till 43rd day (28 days treatment). Later all male animals got cohabited with female animals in the ratio 1:3. On confirmation of mating, female animals were isolated. Male animals were euthanized on batches. Testis and epididymis were weighed and homogenized. Sperm count, motility, morphology, viability, and headcount. The serum collected was evaluated for serum FSH, LH, and testosterone levels. On day Gestational day 15, gravid uterus observations were calculated to evaluate male and female fertility parameters. There were statistically significant improvements (p