Topical and systemic use of Joannesia princeps vell. LC seed oil in acute pain and inflammation induced by different agents

• Published in: Journal of ethnopharmacology 2021-03, Vol.268, p.113554, Article 113554
• Main Authors: Conceição de Souza, Milene, Hubner, Jhones Trindade, Gonçalves, Gabriela Mastrangelo, Ferreira da Costa Fernandes, Thayane, Laureano-Melo, Roberto, da Silva Côrtes, Wellington, Gonçalves, Lenicio, de Assis da Silva, Francisco, Marinho, Bruno Guimarães
• Format: Article
• Language: English
• Subjects: Acute Pain - drug therapy; Acute Pain - metabolism; Administration, Topical; Analgesics - administration & dosage; Analgesics - isolation & purification; Animals; Anti-Inflammatory Agents - administration & dosage; Anti-Inflammatory Agents - isolation & purification; Arachidonic acid; Carrageenan - toxicity; Cytokines; Dose-Response Relationship, Drug; Ear oedema; Edema - chemically induced; Edema - drug therapy; Edema - metabolism; Euphorbiaceae; Inflammation - chemically induced; Inflammation - drug therapy; Inflammation - metabolism; Joannesia princeps, paw oedema; Male; Mice; Pain Measurement - drug effects; Pain Measurement - methods; Plant Extracts - administration & dosage; Plant Extracts - isolation & purification; Plant Oils - administration & dosage; Plant Oils - isolation & purification; Seeds
• ISSN: 0378-8741; 1872-7573
• DOI: 10.1016/j.jep.2020.113554

Joannesia princeps (SOJP) has been used in folk medicine as anthelmintic treatment and cutaneous wound healing. Aim of the study: The purpose of this study is to evaluate the pharmacological activity of seed oil of Joannesia princeps, administered systemically and topically, on acute pain and inflammation. Male swiss mice were treated orally and topically with seed oil of Joannesia princeps in models of acute pain (acetic acid-induced abdominal writhing, formalin-induced licking behaviour and tail flick tests) and acute inflammation (carrageenan- and histamine-induced paw oedema; arachidonic acid-, capsaicin- and croton oil-induced ear oedema and air pouch tests), besides the open field model in the motor performance evaluation. Seed oil of Joannesia princeps showed systemic action against acute pain in abdominal writhing test (37% and 56% inhibition in the number of writhes at doses of 30 and 100 mg/kg, respectively) and in the second phase of formalin-induced licking behaviour test (29%, 47 and 52% inhibition in the licking time at doses of 10, 30 and 100 mg/kg, respectively), as well as reducing croton oil-induced ear oedema by 72%, leukocyte recruitment and production of TNF-α and IL-6 in the air pouch tests. In addition, topical administration of SOJP inhibited carrageenan-induced paw oedema by 39% at dose of 500 μg/paw and inhibited histamine-induced oedema by 43 and 52% at doses of 300 and 500 μg/paw, respectively. SOJP also decreased croton oil-induced ear oedema by 67% at dose of 500 μg/paw and arachidonic acid-induced ear oedema by 63% at dose of 500 μg/paw, reducing the production of TNF-α, IL-1β and MIP2 in both. In addition, no adverse effects were observed at doses up to 2000 mg/kg. Seed oil of Joannesia princeps presents antinociceptive and anti-inflammatory actions through its topical and systemic administration, promoted by inhibition of leukocyte recruitment and cytokine production (TNF-α, IL-1β, IL-6 and MIP-2). [Display omitted] •– SOJP shows peripheral antinociceptive effect against chemical stimulus.•- SOJP shows anti-inflammatory effect by oral and topical routes.•- TNF-α, IL-1β, IL-4, IL-6, MIP-2 and leukocyte recruitment are inhibited by SOJP activity.