Isolation and characterization of anti-inflammatory and analgesic compounds from Uapaca staudtii Pax (Phyllanthaceae) stem bark

Uapaca species including Uapacastaudtii Pax (Phyllanthaceae) are used in West Africa ethnomedicine to treat diverse ailments including pile, rheumatism, oedema and wound healing. However, the anti-inflammatory and analgesic potential as well as constituents of the Uapacastaudtii stem bark has not be...

Full description

Saved in:
Bibliographic Details
Published in:Journal of ethnopharmacology 2021-04, Vol.269, p.113737, Article 113737
Main Authors: Thomas, Paul, Essien, Emmanuel, Udoh, Anwanabasi, Archibong, Bright, Akpan, Ofonasaha, Etukudo, Emediong, De Leo, Marinella, Eseyin, Olorunfemi, Flamini, Guido, Ajibesin, Kola’
Format: Article
Language:English
Subjects:
Betulinic acid
Phyllanthaceae
Protocatechuic acid
Uapacastaudtii
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c353t-2babcda761ab71c4925e4459c3283fe49159bf559de69dda24e6bc6c02a84de33
cites cdi_FETCH-LOGICAL-c353t-2babcda761ab71c4925e4459c3283fe49159bf559de69dda24e6bc6c02a84de33
container_end_page
container_issue
container_start_page 113737
container_title Journal of ethnopharmacology
container_volume 269
creator Thomas, Paul
Essien, Emmanuel
Udoh, Anwanabasi
Archibong, Bright
Akpan, Ofonasaha
Etukudo, Emediong
De Leo, Marinella
Eseyin, Olorunfemi
Flamini, Guido
Ajibesin, Kola’
description Uapaca species including Uapacastaudtii Pax (Phyllanthaceae) are used in West Africa ethnomedicine to treat diverse ailments including pile, rheumatism, oedema and wound healing. However, the anti-inflammatory and analgesic potential as well as constituents of the Uapacastaudtii stem bark has not been investigated. The study was designed to evaluate the anti-inflammatory, analgesic, and antioxidant activities of extract and fractions ofU. staudtii stem bark, and to isolate the bioactive constituents. The anti-inflammatory, analgesic and antioxidant activities of the ethanol extract, dichloromethane, ethyl acetate, butanol, and aqueous fractions of U. staudtii stem bark, as well as protocatechuic acid and betulinic acid isolated from the bioactive ethyl acetate fraction were evaluated in different mice models of inflammation and pain; furthermore, antioxidant assays were carried out. Chemical structures of isolated compounds were established based on spectroscopic studies and comparison with literature data. The ethanol extract and ethyl acetate fraction exhibited good anti-inflammatory, analgesic, and antioxidant capacity in all studied models, comparable with those of the standard drugs used. Protocatechuic acid also gave significant (p 
doi_str_mv 10.1016/j.jep.2020.113737
format article
fullrecord <record><control><sourceid>pubmed_cross</sourceid><recordid>TN_cdi_crossref_primary_10_1016_j_jep_2020_113737</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0378874120336254</els_id><sourcerecordid>33359855</sourcerecordid><originalsourceid>FETCH-LOGICAL-c353t-2babcda761ab71c4925e4459c3283fe49159bf559de69dda24e6bc6c02a84de33</originalsourceid><addsrcrecordid>eNp9kDtPxDAQhC0EguPxA2iQSyhy2HEcJ6JCiJeEBAXU1sbecD6SOLJziKPhrxMIUFKtdnZmtPoIOeRszhnPT5fzJfbzlKXjzoUSaoPMeKHSREklNsmMCVUkhcr4DtmNcckYUzxj22RHCCHLQsoZ-biNvoHB-Y5CZ6lZQAAzYHDvk-jrUR9c4rq6gbaFwYf1txM6aJ4xOkONb3u_6mykdfAtfYIeDNA4wMoOztEHeKPHD4t104xFCzAIeDJesaUVhJd9slVDE_HgZ-6Rp6vLx4ub5O7--vbi_C4xQoohSSuojAWVc6gUN1mZSswyWRqRFqLGrOSyrGopS4t5aS2kGeaVyQ1LocgsCrFH-NRrgo8xYK374FoIa82Z_oKpl3qEqb9g6gnmmDmaMv2qatH-JX7pjYazyYDj568Og47GYWfQuoBm0Na7f-o_AYmph-M</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Isolation and characterization of anti-inflammatory and analgesic compounds from Uapaca staudtii Pax (Phyllanthaceae) stem bark</title><source>Elsevier:Jisc Collections:Elsevier Read and Publish Agreement 2022-2024:Freedom Collection (Reading list)</source><creator>Thomas, Paul ; Essien, Emmanuel ; Udoh, Anwanabasi ; Archibong, Bright ; Akpan, Ofonasaha ; Etukudo, Emediong ; De Leo, Marinella ; Eseyin, Olorunfemi ; Flamini, Guido ; Ajibesin, Kola’</creator><creatorcontrib>Thomas, Paul ; Essien, Emmanuel ; Udoh, Anwanabasi ; Archibong, Bright ; Akpan, Ofonasaha ; Etukudo, Emediong ; De Leo, Marinella ; Eseyin, Olorunfemi ; Flamini, Guido ; Ajibesin, Kola’</creatorcontrib><description>Uapaca species including Uapacastaudtii Pax (Phyllanthaceae) are used in West Africa ethnomedicine to treat diverse ailments including pile, rheumatism, oedema and wound healing. However, the anti-inflammatory and analgesic potential as well as constituents of the Uapacastaudtii stem bark has not been investigated. The study was designed to evaluate the anti-inflammatory, analgesic, and antioxidant activities of extract and fractions ofU. staudtii stem bark, and to isolate the bioactive constituents. The anti-inflammatory, analgesic and antioxidant activities of the ethanol extract, dichloromethane, ethyl acetate, butanol, and aqueous fractions of U. staudtii stem bark, as well as protocatechuic acid and betulinic acid isolated from the bioactive ethyl acetate fraction were evaluated in different mice models of inflammation and pain; furthermore, antioxidant assays were carried out. Chemical structures of isolated compounds were established based on spectroscopic studies and comparison with literature data. The ethanol extract and ethyl acetate fraction exhibited good anti-inflammatory, analgesic, and antioxidant capacity in all studied models, comparable with those of the standard drugs used. Protocatechuic acid also gave significant (p &lt; 0.05) anti-inflammatory (83%and 88% inhibition for egg-albumin induced and xylene induced oedema, respectively), analgesic (56% inhibition and 22 s of pain suppression for acetic acid-induced and hot plate-induced pain, respectively), and antioxidant effects (97% inhibition and absorbance of 2.516 at 100 μg/mL for DPPH and FRAP assay, respectively) in all the models, whereas betulinic acid only exhibited significant (p &lt; 0.05) anti-inflammatory and antioxidant activity. The result supports the medicinal uses of the U. staudtii stem bark in the management of pain and inflammatory disease. This is the first report on the biological activities and characterization of compounds inU. staudtii, and presence of protocatechuic acid in Uapaca genus. [Display omitted] •Protocatechuic acid characterized from Uapaca genus for the first time.•Substantial amount of betulinic acid characterized from Uapaca staudtii stem bark.•Significant anti-inflammatory and analgesic activities exhibited by Uapaca staudtii stem bark.•Uapaca staudtii stem bark and its constituents exhibited remarkable antioxidant effect. .</description><identifier>ISSN: 0378-8741</identifier><identifier>EISSN: 1872-7573</identifier><identifier>DOI: 10.1016/j.jep.2020.113737</identifier><identifier>PMID: 33359855</identifier><language>eng</language><publisher>Ireland: Elsevier B.V</publisher><subject>Betulinic acid ; Phyllanthaceae ; Protocatechuic acid ; Uapacastaudtii</subject><ispartof>Journal of ethnopharmacology, 2021-04, Vol.269, p.113737, Article 113737</ispartof><rights>2020 Elsevier B.V.</rights><rights>Copyright © 2020 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c353t-2babcda761ab71c4925e4459c3283fe49159bf559de69dda24e6bc6c02a84de33</citedby><cites>FETCH-LOGICAL-c353t-2babcda761ab71c4925e4459c3283fe49159bf559de69dda24e6bc6c02a84de33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33359855$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Thomas, Paul</creatorcontrib><creatorcontrib>Essien, Emmanuel</creatorcontrib><creatorcontrib>Udoh, Anwanabasi</creatorcontrib><creatorcontrib>Archibong, Bright</creatorcontrib><creatorcontrib>Akpan, Ofonasaha</creatorcontrib><creatorcontrib>Etukudo, Emediong</creatorcontrib><creatorcontrib>De Leo, Marinella</creatorcontrib><creatorcontrib>Eseyin, Olorunfemi</creatorcontrib><creatorcontrib>Flamini, Guido</creatorcontrib><creatorcontrib>Ajibesin, Kola’</creatorcontrib><title>Isolation and characterization of anti-inflammatory and analgesic compounds from Uapaca staudtii Pax (Phyllanthaceae) stem bark</title><title>Journal of ethnopharmacology</title><addtitle>J Ethnopharmacol</addtitle><description>Uapaca species including Uapacastaudtii Pax (Phyllanthaceae) are used in West Africa ethnomedicine to treat diverse ailments including pile, rheumatism, oedema and wound healing. However, the anti-inflammatory and analgesic potential as well as constituents of the Uapacastaudtii stem bark has not been investigated. The study was designed to evaluate the anti-inflammatory, analgesic, and antioxidant activities of extract and fractions ofU. staudtii stem bark, and to isolate the bioactive constituents. The anti-inflammatory, analgesic and antioxidant activities of the ethanol extract, dichloromethane, ethyl acetate, butanol, and aqueous fractions of U. staudtii stem bark, as well as protocatechuic acid and betulinic acid isolated from the bioactive ethyl acetate fraction were evaluated in different mice models of inflammation and pain; furthermore, antioxidant assays were carried out. Chemical structures of isolated compounds were established based on spectroscopic studies and comparison with literature data. The ethanol extract and ethyl acetate fraction exhibited good anti-inflammatory, analgesic, and antioxidant capacity in all studied models, comparable with those of the standard drugs used. Protocatechuic acid also gave significant (p &lt; 0.05) anti-inflammatory (83%and 88% inhibition for egg-albumin induced and xylene induced oedema, respectively), analgesic (56% inhibition and 22 s of pain suppression for acetic acid-induced and hot plate-induced pain, respectively), and antioxidant effects (97% inhibition and absorbance of 2.516 at 100 μg/mL for DPPH and FRAP assay, respectively) in all the models, whereas betulinic acid only exhibited significant (p &lt; 0.05) anti-inflammatory and antioxidant activity. The result supports the medicinal uses of the U. staudtii stem bark in the management of pain and inflammatory disease. This is the first report on the biological activities and characterization of compounds inU. staudtii, and presence of protocatechuic acid in Uapaca genus. [Display omitted] •Protocatechuic acid characterized from Uapaca genus for the first time.•Substantial amount of betulinic acid characterized from Uapaca staudtii stem bark.•Significant anti-inflammatory and analgesic activities exhibited by Uapaca staudtii stem bark.•Uapaca staudtii stem bark and its constituents exhibited remarkable antioxidant effect. .</description><subject>Betulinic acid</subject><subject>Phyllanthaceae</subject><subject>Protocatechuic acid</subject><subject>Uapacastaudtii</subject><issn>0378-8741</issn><issn>1872-7573</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNp9kDtPxDAQhC0EguPxA2iQSyhy2HEcJ6JCiJeEBAXU1sbecD6SOLJziKPhrxMIUFKtdnZmtPoIOeRszhnPT5fzJfbzlKXjzoUSaoPMeKHSREklNsmMCVUkhcr4DtmNcckYUzxj22RHCCHLQsoZ-biNvoHB-Y5CZ6lZQAAzYHDvk-jrUR9c4rq6gbaFwYf1txM6aJ4xOkONb3u_6mykdfAtfYIeDNA4wMoOztEHeKPHD4t104xFCzAIeDJesaUVhJd9slVDE_HgZ-6Rp6vLx4ub5O7--vbi_C4xQoohSSuojAWVc6gUN1mZSswyWRqRFqLGrOSyrGopS4t5aS2kGeaVyQ1LocgsCrFH-NRrgo8xYK374FoIa82Z_oKpl3qEqb9g6gnmmDmaMv2qatH-JX7pjYazyYDj568Og47GYWfQuoBm0Na7f-o_AYmph-M</recordid><startdate>20210406</startdate><enddate>20210406</enddate><creator>Thomas, Paul</creator><creator>Essien, Emmanuel</creator><creator>Udoh, Anwanabasi</creator><creator>Archibong, Bright</creator><creator>Akpan, Ofonasaha</creator><creator>Etukudo, Emediong</creator><creator>De Leo, Marinella</creator><creator>Eseyin, Olorunfemi</creator><creator>Flamini, Guido</creator><creator>Ajibesin, Kola’</creator><general>Elsevier B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20210406</creationdate><title>Isolation and characterization of anti-inflammatory and analgesic compounds from Uapaca staudtii Pax (Phyllanthaceae) stem bark</title><author>Thomas, Paul ; Essien, Emmanuel ; Udoh, Anwanabasi ; Archibong, Bright ; Akpan, Ofonasaha ; Etukudo, Emediong ; De Leo, Marinella ; Eseyin, Olorunfemi ; Flamini, Guido ; Ajibesin, Kola’</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c353t-2babcda761ab71c4925e4459c3283fe49159bf559de69dda24e6bc6c02a84de33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Betulinic acid</topic><topic>Phyllanthaceae</topic><topic>Protocatechuic acid</topic><topic>Uapacastaudtii</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Thomas, Paul</creatorcontrib><creatorcontrib>Essien, Emmanuel</creatorcontrib><creatorcontrib>Udoh, Anwanabasi</creatorcontrib><creatorcontrib>Archibong, Bright</creatorcontrib><creatorcontrib>Akpan, Ofonasaha</creatorcontrib><creatorcontrib>Etukudo, Emediong</creatorcontrib><creatorcontrib>De Leo, Marinella</creatorcontrib><creatorcontrib>Eseyin, Olorunfemi</creatorcontrib><creatorcontrib>Flamini, Guido</creatorcontrib><creatorcontrib>Ajibesin, Kola’</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Journal of ethnopharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Thomas, Paul</au><au>Essien, Emmanuel</au><au>Udoh, Anwanabasi</au><au>Archibong, Bright</au><au>Akpan, Ofonasaha</au><au>Etukudo, Emediong</au><au>De Leo, Marinella</au><au>Eseyin, Olorunfemi</au><au>Flamini, Guido</au><au>Ajibesin, Kola’</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation and characterization of anti-inflammatory and analgesic compounds from Uapaca staudtii Pax (Phyllanthaceae) stem bark</atitle><jtitle>Journal of ethnopharmacology</jtitle><addtitle>J Ethnopharmacol</addtitle><date>2021-04-06</date><risdate>2021</risdate><volume>269</volume><spage>113737</spage><pages>113737-</pages><artnum>113737</artnum><issn>0378-8741</issn><eissn>1872-7573</eissn><abstract>Uapaca species including Uapacastaudtii Pax (Phyllanthaceae) are used in West Africa ethnomedicine to treat diverse ailments including pile, rheumatism, oedema and wound healing. However, the anti-inflammatory and analgesic potential as well as constituents of the Uapacastaudtii stem bark has not been investigated. The study was designed to evaluate the anti-inflammatory, analgesic, and antioxidant activities of extract and fractions ofU. staudtii stem bark, and to isolate the bioactive constituents. The anti-inflammatory, analgesic and antioxidant activities of the ethanol extract, dichloromethane, ethyl acetate, butanol, and aqueous fractions of U. staudtii stem bark, as well as protocatechuic acid and betulinic acid isolated from the bioactive ethyl acetate fraction were evaluated in different mice models of inflammation and pain; furthermore, antioxidant assays were carried out. Chemical structures of isolated compounds were established based on spectroscopic studies and comparison with literature data. The ethanol extract and ethyl acetate fraction exhibited good anti-inflammatory, analgesic, and antioxidant capacity in all studied models, comparable with those of the standard drugs used. Protocatechuic acid also gave significant (p &lt; 0.05) anti-inflammatory (83%and 88% inhibition for egg-albumin induced and xylene induced oedema, respectively), analgesic (56% inhibition and 22 s of pain suppression for acetic acid-induced and hot plate-induced pain, respectively), and antioxidant effects (97% inhibition and absorbance of 2.516 at 100 μg/mL for DPPH and FRAP assay, respectively) in all the models, whereas betulinic acid only exhibited significant (p &lt; 0.05) anti-inflammatory and antioxidant activity. The result supports the medicinal uses of the U. staudtii stem bark in the management of pain and inflammatory disease. This is the first report on the biological activities and characterization of compounds inU. staudtii, and presence of protocatechuic acid in Uapaca genus. [Display omitted] •Protocatechuic acid characterized from Uapaca genus for the first time.•Substantial amount of betulinic acid characterized from Uapaca staudtii stem bark.•Significant anti-inflammatory and analgesic activities exhibited by Uapaca staudtii stem bark.•Uapaca staudtii stem bark and its constituents exhibited remarkable antioxidant effect. .</abstract><cop>Ireland</cop><pub>Elsevier B.V</pub><pmid>33359855</pmid><doi>10.1016/j.jep.2020.113737</doi></addata></record>
fulltext fulltext
identifier ISSN: 0378-8741
ispartof Journal of ethnopharmacology, 2021-04, Vol.269, p.113737, Article 113737
issn 0378-8741
1872-7573
language eng
recordid cdi_crossref_primary_10_1016_j_jep_2020_113737
source Elsevier:Jisc Collections:Elsevier Read and Publish Agreement 2022-2024:Freedom Collection (Reading list)
subjects Betulinic acid
Phyllanthaceae
Protocatechuic acid
Uapacastaudtii
title Isolation and characterization of anti-inflammatory and analgesic compounds from Uapaca staudtii Pax (Phyllanthaceae) stem bark
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-07T07%3A44%3A58IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-pubmed_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Isolation%20and%20characterization%20of%20anti-inflammatory%20and%20analgesic%20compounds%20from%20Uapaca%20staudtii%20Pax%20(Phyllanthaceae)%20stem%20bark&rft.jtitle=Journal%20of%20ethnopharmacology&rft.au=Thomas,%20Paul&rft.date=2021-04-06&rft.volume=269&rft.spage=113737&rft.pages=113737-&rft.artnum=113737&rft.issn=0378-8741&rft.eissn=1872-7573&rft_id=info:doi/10.1016/j.jep.2020.113737&rft_dat=%3Cpubmed_cross%3E33359855%3C/pubmed_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c353t-2babcda761ab71c4925e4459c3283fe49159bf559de69dda24e6bc6c02a84de33%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_id=info:pmid/33359855&rfr_iscdi=true