Evaluation of Two Protocols Using Autologous Conditioned Serum for Intra-articular Therapy of Equine Osteoarthritis—A Pilot Study Monitoring Cytokines and Cartilage-Specific Biomarkers

• Published in: Journal of equine veterinary science 2018-01, Vol.60, p.35-42.e2
• Main Authors: Lasarzik, Juliane, Bondzio, Angelika, Rettig, Matthias, Estrada, Roberto, Klaus, Christoph, Ehrle, Anna, Einspanier, Ralf, Lischer, Christoph Johannes
• Format: Article
• Language: English
• Subjects: Autologous conditioned serum; C12C; CP II; CS 846; IL-1ra; IL-1β
• ISSN: 0737-0806; 1542-7412
• DOI: 10.1016/j.jevs.2016.09.014

We hypothesised that shorter treatment intervals of intraarticular autologous conditioned serum (ACS) injections would more beneficially affect the synovial fluid (SF) concentrations of IL-1ra, IL-1β and cartilage biomarkers, compared with the traditional weekly treatment intervals in joints suffering from natural OA. In a randomised comparative study, 12 horses with OA were allocated to two groups (n = 6). The horses in group 1 received three intraarticular ACS injections at weekly intervals, whereas the horses in group 2 received three intraarticular ACS injections at two-day intervals. The levels of IL-1ra, IL-1β, CPII, C12C and CS 846 were determined in SF before and after ACS treatment using commercially available ELISA kits. The SF IL-1ra concentration 1 hour and 4 hours after ACS injection was significantly increased compared to baseline levels and decreased back to it within 48 hours. Comparing the SF IL-1ra, IL-1β, C12C, CS 846 and CP II levels before and 42 days after ACS treatment, group 2 showed a significant decrease in all parameters and an approximation on the levels in normal joints. These results indicate that the long-time effect of an ACS treatment given at two-day intervals is characterized by decreased SF IL1ra, IL-1β, C12C, CP II and CS 846 concentrations, which might indicate an improvement in joint inflammation and cartilage degrading processes . Further investigations with greater sample sizes have to prove if the two-day treatment interval is preferable to the widely used treatment protocol of weekly intraarticular ACS injections. •SF IL-1ra increased 1 hour and 4 hours after ACS injection.•SF IL-1ra decreased back to baseline levels within 48 hours after ACS injection.•SF IL-1ra after ACS injection was positively correlated with IL-1ra in the ACS.•All SF parameters approximated on levels in normal joints after a two-day ACS treatment interval.