A reversed-phase high-performance liquid chromatography method combined with matrix solid-phase dispersion extraction for the determination of teflubenzuron, lufenuron and bifenthrin residues in lyophilized coconut water

• Published in: Journal of food composition and analysis 2012-05, Vol.26 (1-2), p.183-188
• Main Authors: Santos, Luis Fabrício Santana, Souza, Nicaellen Roberta da Silva, Ferreira, Jordana Alves, Navickiene, Sandro
• Format: Article
• Language: English
• Subjects: Biological and medical sciences; Coconut water; Cocos nucifera; Food analysis; Food composition; Food engineering; Food industries; Food safety; Fundamental and applied biological sciences. Psychology; General aspects; HPLC; Liquid chromatography/UV–vis absorbance detector; Lyophilization; Matrix solid-phase dispersion; Methods of analysis, processing and quality control, regulation, standards; Pesticides
• ISSN: 0889-1575; 1096-0481
• DOI: 10.1016/j.jfca.2012.03.007

► Method to analyze lyophilized coconut water pesticides reported for the first time. ► HPLC analysis of pyrethroid and benzoylurea residues in lyophilized coconut water. ► Use of MSPD extraction method to determine pesticides in lyophilized coconut water. An extraction method based on matrix solid-phase dispersion was developed to determine lufenuron, bifenthrin and teflubenzuron in lyophilized coconut water using liquid chromatography with UV–vis absorbance detector. The best results were obtained using 0.5g of lyophilized coconut water, 2.5g of C18-bonded silica as dispersant sorbent and acetonitrile (20mL) as eluting solvent. The method was validated using lyophilized coconut water samples fortified with pesticides at three concentration levels (0.25, 0.5 and 1.0mgkg−1). Average recoveries (3 replicates) ranged from 74 to 116%, with relative standard deviations between 0.4 and 9.4%. Detection limits for lyophilized coconut water ranged from 0.04 to 0.05mgkg−1 and quantification limit was of 0.1mgkg−1.