Synthesis and surface properties of new semi-fluorinated sulfobetaines potentially usable for 2D-electrophoresis

Semi-fluorinated counterparts (FASB- p, m) of amidosulfobetaines ASB-n were synthesized, their surface properties compared and their great potentiality in proteomic analysis demonstrated using 2D electrophoresis. ▪ New semi-fluorinated amidosulfobetaines, homologs of hydrocarbon amidosulfobetaines (...

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Bibliographic Details
Published in:Journal of fluorine chemistry 2007-03, Vol.128 (3), p.211-218
Main Authors: Thebault, Pascal, Taffin de Givenchy, Elisabeth, Starita-Geribaldi, Mireille, Guittard, Frederic, Geribaldi, Serge
Format: Article
Language:English
Subjects:
2D electrophoresis
Critical micelle concentration
Semi-fluorinated
Sulfobetaine
Surface tension
Surfactant
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Summary:Semi-fluorinated counterparts (FASB- p, m) of amidosulfobetaines ASB-n were synthesized, their surface properties compared and their great potentiality in proteomic analysis demonstrated using 2D electrophoresis. ▪ New semi-fluorinated amidosulfobetaines, homologs of hydrocarbon amidosulfobetaines (ASB) commonly used in two-dimensional gel electrophoresis (2DE), were prepared in three steps from 2-F-alkylethyl iodide or F-alkyl iodide. Their synthesis was described and their air–water interface properties were investigated and compared with their perhydrogenated counterpart properties. The influence of the relative lengths of the perfluorinated and hydocarbonated moieties was discussed. 2DE of a rat testicular membrane fraction was performed comparatively using one of these fluorinated sulfobetaines and its hydrocarbon homolog; these preliminary results showed the great potential of the semi-fluorinated sulfobetaines in proteomic analysis.
ISSN:0022-1139
1873-3328
DOI:10.1016/j.jfluchem.2006.12.008