Investigation of anti-bacterial activity and cytotoxicity of calcium fluoride nanoparticles

[Display omitted] •CaF2 Nps isolated using co-precipitation method was characterized using XRD, FESEM, AFM, Zeta potential measurements, etc.•The antibacterial activity of CaF2 Nps against both Gram negative and Gram positive bacterial strains was tested.•The MIC values of CaF2 Nps evaluated by turb...

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Bibliographic Details
Published in:Journal of fluorine chemistry 2017-01, Vol.193, p.38-44
Main Authors: Bala, Whitelin Annie, Benitha, V.S., Jeyasubramanian, K., Hikku, G.S., Sankar, Padmavathy, Kumar, S. Vignesh
Format: Article
Language:English
Subjects:
Antibacterial agent
Calcium fluoride nanoparticles
Cyto-toxicity
Human pathogens
MIC
MTT assay
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Summary:[Display omitted] •CaF2 Nps isolated using co-precipitation method was characterized using XRD, FESEM, AFM, Zeta potential measurements, etc.•The antibacterial activity of CaF2 Nps against both Gram negative and Gram positive bacterial strains was tested.•The MIC values of CaF2 Nps evaluated by turbidimetric assay against selected bacteria were found to be very low.•Further, cytotoxicity of CaF2 Nps was determined by MTT assay and show non-toxic behaviour towards eukaryotic Vero cells.•Selective toxicity of CaF2 Nps towards prokaryotic bacteria suggests its potential application as antibacterial agent. This paper details about the isolation of Calcium fluoride nanoparticles (CaF2 Nps) through facile co-precipitation method. As-obtained nanoparticles were characterized using XRD, FESEM, AFM, Zeta potential measurements, etc. Further, we evaluated the antibacterial activity of CaF2 Nps against both gram negative (Escherichia coli, Pseudomonas aeruginosa) and gram positive (Bacillus badius, Staphylococcus aureus) bacterial strains. The in-vitro toxicity of CaF2 Nps to mammalian cells, normal African green monkey Chlorocebus kidney epithelial cell line (Vero) was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and characterized using phase contrast microscopy. The minimum inhibitory concentration (MIC) values of these nanoparticles towards the prokaryotic micro-organisms were found to be considerably lower than many other nanoparticles and antibiotics. Further the nanoparticles were found to be nontoxic against eukaryotic normal Vero cells. The selective toxicity of CaF2 Nps to prokaryotic bacterial pathogen compared to eukaryotic cell suggests that CaF2 Nps can be used as an excellent antibacterial agent.
ISSN:0022-1139
1873-3328
DOI:10.1016/j.jfluchem.2016.11.014