Development of immobilized lysozyme based active film

The aim of this work is to develop an active packaging material in which the active compound, lysozyme, is completely immobilized onto the polymeric material and acts directly from the film without being released into the packed foodstuff. Cross-linked films of polyvinyl alcohol, differing in the am...

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Bibliographic Details
Published in:Journal of food engineering 2007-02, Vol.78 (3), p.741-745
Main Authors: Conte, A., Buonocore, G.G., Sinigaglia, M., Del Nobile, M.A.
Format: Article
Language:English
Subjects:
Active packaging
antimicrobial properties
coatings
crosslinking
films (materials)
food packaging
Immobilization
immobilized enzymes
Lysozyme
new products
packaging materials
poly(vinyl alcohol)
polymers
product development
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Summary:The aim of this work is to develop an active packaging material in which the active compound, lysozyme, is completely immobilized onto the polymeric material and acts directly from the film without being released into the packed foodstuff. Cross-linked films of polyvinyl alcohol, differing in the amount of both antimicrobial and binding agents, were prepared. In order to determine the amount of active substance completely bonded to the polymer backbone, lysozyme release tests were run. The antimicrobial activity of the developed active films was investigated in order to verify their effectiveness in controlling microbial spoilage. A modified version of Gompertz equation was used to quantitatively determine the antimicrobial activity of the films. Results indicate the developed active films are effective in inhibiting the growth of selected microorganism, and that the antimicrobial activity of the investigated films increases as the amount of enzyme incorporated increases.
ISSN:0260-8774
1873-5770
DOI:10.1016/j.jfoodeng.2005.11.013