RNA Guided Genome Editing in Mouse Germ-Line Stem Cells

Spermatogonial stem cells （SSCs） reside on the basement membrane of the seminiferous tubules in mammalian testes （Nagano et al., 1998）. After isolation and purification of SSCs from mouse testis, SSCs can be cultured in vitro to derive germ-line stem cells （GSCs） which have the ability of proliferat...

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Bibliographic Details
Published in:Journal of genetics and genomics 2014-07, Vol.41 (7), p.409-411
Main Authors: Wang, Xuepeng, Yuan, Yan, Zhou, Quan, Wan, Haifeng, Wang, Mei, Zhou, Qi, Zhao, Xiao-Yang, Sha, Jiahao
Format: Article
Language:English
Subjects:
Animals
A基因组
Base Sequence
Genetic Engineering - methods
Male
Mice
RNA, Guide, CRISPR-Cas Systems
Spermatozoa - cytology
Stem Cells - metabolism
Testis - cytology
体外培养
哺乳动物
小鼠睾丸
曲细精管
种系
精原干细胞
编辑
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Description
Summary:Spermatogonial stem cells （SSCs） reside on the basement membrane of the seminiferous tubules in mammalian testes （Nagano et al., 1998）. After isolation and purification of SSCs from mouse testis, SSCs can be cultured in vitro to derive germ-line stem cells （GSCs） which have the ability of proliferation over 2 years （Kanatsu-Shinohara et al.. 2003;
ISSN:1673-8527
DOI:10.1016/j.jgg.2014.06.001