Decolourization and detoxification of textile industry wastewater by the laccase-mediator system

Decolourization and detoxification of a textile industry effluent by laccase from Trametes trogii in the presence and the absence of laccase mediators was investigated. Laccase alone was not able to decolourize the effluent efficiently even at the highest enzyme concentration tested: less than 10% d...

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Bibliographic Details
Published in:Journal of hazardous materials 2010-03, Vol.175 (1), p.802-808
Main Authors: Khlifi, Rim, Belbahri, Lassad, Woodward, Steve, Ellouz, Mariem, Dhouib, Abdelhafidh, Sayadi, Sami, Mechichi, Tahar
Format: Article
Language:English
Subjects:
Applied sciences
Biodegradation, Environmental
Bioreactors
Coloring Agents
Dye
Exact sciences and technology
General purification processes
Hydrogen-Ion Concentration
Industrial Waste
Laccase
Laccase - chemistry
Mediator
Pollution
Temperature
Textile Industry
Textiles
Trametes - metabolism
Waste Disposal, Fluid
Wastewater
Wastewaters
Water Pollutants, Chemical - chemistry
Water Pollutants, Chemical - isolation & purification
Water Purification - methods
Water treatment and pollution
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Summary:Decolourization and detoxification of a textile industry effluent by laccase from Trametes trogii in the presence and the absence of laccase mediators was investigated. Laccase alone was not able to decolourize the effluent efficiently even at the highest enzyme concentration tested: less than 10% decolourization was obtained with 9 U/mL reaction mixture. To enhance effluent decolourization, several potential laccase mediators were tested at concentrations ranging from 0 to 1 mM. Most potential mediators enhanced decolourization of the effluent, with 1-hydroxybenzotriazol (HBT) being the most effective. The effect of several physico-chemical parameters that could influence enzyme activity, such as pH, temperature and dye concentration was tested. Optimal decolourization occurred with 20% effluent at pH 5, a temperature of 50 °C, and in the presence of 1 mM HBT. The toxicities of crude, laccase–HBT treated and laccase–acetosyringone treated effluent were evaluated using the Microtox assay. Only laccase–acetosyringone treated effluent was not toxic; crude and laccase–HBT treated effluent retained toxicity.
ISSN:0304-3894
1873-3336
DOI:10.1016/j.jhazmat.2009.10.079