Enhanced anaerobic digestion performance by two artificially constructed microbial consortia capable of woody biomass degradation and chlorophenols detoxification

• Published in: Journal of hazardous materials 2020-05, Vol.389, p.122076, Article 122076
• Main Authors: Ali, Sameh S., Kornaros, Michael, Manni, Alessandro, Sun, Jianzhong, El-Shanshoury, Abd El-Raheem R., Kenawy, El-Refaie, Khalil, Maha A.
• Format: Article
• Language: English
• Subjects: Aliivibrio fischeri - drug effects; Anaerobic digestion; Anaerobiosis; Animals; Archaea - metabolism; Bacteria - metabolism; Biodegradation and detoxification; Biodegradation, Environmental; Biofuels; Biomass; Bioreactors; Chlorophenols; Chlorophenols - metabolism; Chlorophenols - toxicity; Daphnia - drug effects; Lactuca - drug effects; Lignin - metabolism; Methane - biosynthesis; Methanogenic Archaea; Microbial Consortia - physiology; Microbial consortium; Persistent Organic Pollutants - metabolism; Persistent Organic Pollutants - toxicity; Wood - metabolism; Woody biomass
• ISSN: 0304-3894; 1873-3336
• DOI: 10.1016/j.jhazmat.2020.122076

[Display omitted] •CS-5 and BC-4 constructed for lignocellulose deconstruction and chlorophenols detoxification.•The achieved weight loss of the CSW induced by CS-5 and BC-4 reached 69.2 and 56.3 %, respectively.•CS-5 and BC-4 would potentially serve as a useful agent for detoxifying CPs.•The synergistic action of CS-5 and BC-4 increased CH4 production by 113.7 % at the AD peak phase.•Methanosataceae represented 45.1 % of the methanogenic Archaea. Catalpa sawdust (CSW) is a promising biomass-based biofuel. However, the complex lignocellulosic structure limits its efficient utilization in biorefinery applications. It is even more so when chlorophenols (CPs), highly toxic organic substances widely used as wood preservatives, are present. Hence, it is crucial to develop effective and eco-friendly approaches to attain deconstruction of lignocellulose and chlorophenols simultaneously as well as to improve methane (CH4) production efficiently. This study might be the first to explore the performance of the novel constructed microbial consortia CS-5 and BC-4 on woody biomass degradation and CPs detoxification simultaneously with CH4 production. After the degradation of CSW and CPs for 15 days by C5-5 or BC-4, significant reduction in lignocellulosic components and CPs mixture was realized with a total weight loss of 69.2 and 56.3 % and CPs degradation of 89 and 95 %, respectively. The toxicity of individual or mixed CPs after 15 days of degradation was reduced by approximately 90 %. The synergistic action of CS-5 and BC-4 enhanced biogas and CH4 yields over 76 and 64 % respectively, higher than control. Furthermore, CH4 production increased by 113.7 % at the peak phase of AD process. Methanosataceae represented 45.1 % of the methanogenic Archaea in digester G-III.