Fungal laccase-triggered 17β-estradiol humification kinetics and mechanisms in the presence of humic precursors

• Published in: Journal of hazardous materials 2021-06, Vol.412, p.125197, Article 125197
• Main Authors: Liu, Qingzhu, Liu, Jie, Hong, Dan, Sun, Kai, Li, Shunyao, Latif, Abdul, Si, Xiongyuan, Si, Youbin
• Format: Article
• Language: English
• Subjects: 17β-estradiol; Humic precursors; Humification mechanism; Inhibition effect; Trametes versicolor laccase
• ISSN: 0304-3894; 1873-3336
• DOI: 10.1016/j.jhazmat.2021.125197

Naturally-occurring phenolic acids (PAs) act as humic precursors that participate in the conversion behaviors and coupling pathways of steroidal estrogens (SEs) during laccase-triggered humification processes (L-THPs). Herein, the influences and mechanisms of PAs on Trametes versicolor laccase-evoked 17β-estradiol (E2) conversion kinetics and humification routes were explored. Fungal laccase was fleet in converting > 99% of E2, and the calculated pseudo-first-order velocity constant and half-time values were respectively 0.039 min−1 and 17.906 min. PAs containing an O-dihydroxy moiety such as gallic acid and caffeic acid evidently hampered E2 humification owning to the yielded highly reactive O-quinones reversed E2 radicals by hydrogen transfer mechanism, implying that the inhibition effect was enormously dependent upon the number and position of the phenolic –OH present in humic precursors. Oligomers and polymers with carbon-carbon/oxygen links were tentatively found as E2 main humified species resulting from laccase-evoked successive oxidative-coupling. Note that PAs participating in the humification also encountered oxydehydrogenation, self-polymerization, and cross-binding to E2. Interestingly, the –COOH and –OCH3 groups of PAs could be deprived in radical-caused self/co-polymerization. The generation of humified products not only circumvented the environmental risks of parent compounds but accelerated global carbon sequestration. To our knowledge, this is the first in-depth revelation of the humification pathways and related mechanisms of SEs with humic precursors in aquatic ecosystems by L-THPs. [Display omitted] •The conversion efficiency of E2 with PA-free reached to 99.24% during L-THPs.•PAs containing an O-dihydroxy moiety reversed E2 radicals via forming O-quinones.•Oligomers/polymers with carbon-carbon/oxygen links were discovered as E2 principal products.•PAs participating in L-THPs encountered oxydehydrogenation, self-coupling, and cross-binding to E2.•Humification routes and mechanisms of E2 with humic precursors in L-THPs were proposed.