Targeted mesoporous silica nanoparticles for improved inhibition of disinfectant resistant Listeria monocytogenes and lower environmental pollution

• Published in: Journal of hazardous materials 2021-09, Vol.418, p.126364, Article 126364
• Main Authors: Sudagidan, Mert, Yildiz, Gulsah, Onen, Selin, Al, Rabia, Temiz, Şevval Nur, Yurt, Mediha Nur Zafer, Tasbasi, Behiye Busra, Acar, Elif Esma, Coban, Aysen, Aydin, Ali, Dursun, Ali D., Ozalp, Veli C.
• Format: Article
• Language: English
• Subjects: Aptamers; Disinfectants; Listeria; Nanoparticles; Resistant pathogen bacteria
• ISSN: 0304-3894; 1873-3336
• DOI: 10.1016/j.jhazmat.2021.126364

Benzalkonium chloride (BAC) is a common ingredient of disinfectants used for industrial, medical, food safety and domestic applications. It is a common pollutant detected in surface and wastewaters to induce adverse effects on Human health as well as aquatic and terrestrial life forms. Since disinfectant use is essential in combatting against microorganisms, the best approach to reduce ecotoxicity level is to restrict BAC use. We report here that encapsulation of BAC in mesoporous silica nanoparticles can provide an efficient strategy for inhibition of microbial activity with lower than usual concentrations of disinfectants. As a proof-of-concept, Listeria monocytogenes was evaluated for minimum inhibitory concentration (MIC) of nanomaterial encapsulated BAC. Aptamer molecular gate structures provided a specific targeting of the disinfectant to Listeria cells, leading to high BAC concentrations around bacterial cells, but significantly reduced amounts in total. This strategy allowed to inhibition of BAC resistant Listeria strains with 8 times less the usual disinfectant dose. BAC encapsulated and aptamer functionalized silica nanoparticles (AptBACNP) effectively killed only target bacteria L. monocytogenes, but not the non-target cells, Staphylococcus aureus or Escherichia coli. AptBACNP was not cytotoxic to Human cells as determined by in vitro viability assays. [Display omitted] •Targeted benzalkonium chloride reduces MIC values for resistant Listeria strains.•Mesoporous silica and aptamers gates can be used for efficient targeted disinfection.•Targeted benzalkonium chloride is not cytotoxic when delivered in silica nanoparticles.