Clinical and molecular analysis of combined hepatocellular-cholangiocarcinomas

Combined hepatocellular-cholangiocarcinoma (HCC-CC) show dual hepatocellular and biliary epithelial differentiation. To better understand the relations between cholangiocarcinoma (CC), HCC-CC and hepatocellular carcinoma (HCC), we screened for genetic alterations. A series of nine CC, 15 HCC-CC and...

Full description

Saved in:
Bibliographic Details
Published in:Journal of hepatology 2004-08, Vol.41 (2), p.292-298
Main Authors: Cazals-Hatem, Dominique, Rebouissou, Sandra, Bioulac-Sage, Paulette, Bluteau, Olivier, Blanché, Hélène, Franco, Dominique, Monges, Geneviève, Belghiti, Jacques, Sa Cunha, Antonio, Laurent-Puig, Pierre, Degott, Claude, Zucman-Rossi, Jessica
Format: Article
Language:English
Subjects:
Aged
Alleles
beta Catenin
Biological and medical sciences
Carcinoma, Hepatocellular - genetics
Carcinoma, Hepatocellular - pathology
Cholangiocarcinoma - genetics
Cholangiocarcinoma - pathology
Chromosomal Instability
Chromosomes, Human, Pair 14 - genetics
Chromosomes, Human, Pair 3 - genetics
Cytoskeletal Proteins - genetics
Female
Gastroenterology. Liver. Pancreas. Abdomen
Gene Deletion
Gene mutation
Genes, p53
Genome, Human
Hepatocellular carcinoma
Humans
Liver Neoplasms - genetics
Liver Neoplasms - pathology
Liver. Biliary tract. Portal circulation. Exocrine pancreas
Loss of Heterozygosity
Male
Medical sciences
Microsatellite Repeats
Middle Aged
Mutation
p53
Trans-Activators - genetics
Tumors
β-catenin
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Combined hepatocellular-cholangiocarcinoma (HCC-CC) show dual hepatocellular and biliary epithelial differentiation. To better understand the relations between cholangiocarcinoma (CC), HCC-CC and hepatocellular carcinoma (HCC), we screened for genetic alterations. A series of nine CC, 15 HCC-CC and three separated HCC and CC lesions (‘collision tumors’) were screened for loss of heterozygosity (LOH) using 400 microsatellite markers and for p53 and β-catenin mutations. A comparison with a previously characterized series of 137 HCC was performed. In six cases of CC and HCC-CC, we identified TP53 gene mutations. A CTNNB1/β-catenin was identified in two patients presenting collision tumors, but no mutations were found in CC or in HCC-CC. A high level of chromosome instability in both CC and HCC-CC was found. Recurrent specific LOH were identified at 3p and 14q in more than 50% of the CC and the HCC-CC cases, whereas these chromosomal regions were deleted in less than 10% of the HCC cases ( P
ISSN:0168-8278
1600-0641
DOI:10.1016/j.jhep.2004.04.030