Growth of non-enzymatic cholesterol biosensor using TiO2 decorated graphene oxide with bare GCE and PPy-GCE

• Published in: Journal of the Indian Chemical Society 2023-03, Vol.100 (3), p.100906, Article 100906
• Main Authors: Deivanayaki, S., Jayamurugan, P., Ashokan, S., Gopala Krishnan, V., Yogeswari, B., Ubaidullah, Mohd, Pandit, Bidhan, Sarma, G.V.S.S., Narsetti, Hemanth Kumar
• Format: Article
• Language: English
• Subjects: Chemical oxidative polymerization; Clinical analysis; Nanomaterials; Non-enzymatic
• ISSN: 0019-4522
• DOI: 10.1016/j.jics.2023.100906

In this work, we report an electrochemical cholesterol biosensor based on cholesterol oxidase(ChOx) enzyme immobilized on TiO2- nanoparticles – reduced graphene oxide(rGO) – polypyrrole (PPy) nanocomposite modified electrode.The electrochemical properties of GCE modified PPy (PPy-GCE) were studied using CV (Cyclic Voltammetry) and DPV (Differential Pulse Voltammetry). The developed sensor exhibited piecewise linearity from 0.1 μM to 1 μM and from 1 μM to 600 μM with the sensitivity of 61.665 and 0.1466 (2 mA mM × cm) respectively. The limit of detection of the sensor was found to be 32 nm. Fig. Cyclic Voltammogram for the detection of 100 μM cholestrol in phosphate buffer (7.45 pH) on bare GCE, rGO/GCE and PPY/GCE. [Display omitted] •The detection of cholestrol on bare GCE,andPPY/GCE was done using cyclic voltammetry.•Fig. exhibits the cyclic voltammogram of 100 μM cholestrol and the oxidation peak was observed at 0.685 V for bare GCE and 0.619 V for PPY/GCE.•This shows that the modified electrode shows better sensitivity and conductivity for the detection of cholesterol.•As far as our knowledge, metal oxide decorated reduced graphene oxide was not employed in the detection of Cholestrol.