Thiosemicarbazone-based Dioxomolybdenum (VI) complexes as inorganic nucleases

• Published in: Journal of the Indian Chemical Society 2024-11, Vol.101 (11), p.101361, Article 101361
• Main Authors: Pandey, Niket, Krishnan, Deepa, Suresh, Anushree, Abraham, Jayanthi, Sheela, Angappan
• Format: Article
• Language: English
• Subjects: Dioxomolybdenum(VI) complex; DNA binding; Gel-electrophoresis; Schiff base; Thiosemicarbazones
• ISSN: 0019-4522
• DOI: 10.1016/j.jics.2024.101361

This study focuses on the design of alkyl-substituted thiosemicarbazones from 5-bromosalicylaldehyde and substituted thiosemicarbazides and the corresponding dioxomolybdenum(VI) complexes, hitherto unreported. In addition to elemental analyses, the synthesized ligands and the corresponding complexes are further analyzed by physico-chemical and spectroscopic techniques. The DNA interaction studies are based on UV absorption titration and gel electrophoretic methods. The binding constants (Kb) for the ligands (L1-L4) are found to be 1.20 ± 0.1 × 104 M−1; 1.32 ± 0.2 × 104 M−1; 1.27 ± 0.3 × 104 M−1 and 1.25 ± 0.3 × 104 M−1 respectively and that of the complexes (C1–C4) are 1.45 ± 0.2 × 104 M−1; 2.10 ± 0.4 × 104 M−1; 1.54 ± 0.2 × 104 M−1 and 2.13 ± 0.2 × 104 M−1, respectively. The complexes have shown relatively higher binding propensity as compared to that of the ligands. Further, as the outcome of gel electrophoresis studies, the ligands show a cleavage pattern of nicked circular (Form I). However, the complexes show cleavage patterns of both nicked circular and supercoiled, Forms I & II respectively. This implies that both the ligands and complexes possess varying binding efficacy with DNA and accordingly the cleavage patterns also differ. [Display omitted] •Designed alkyl-substituted thiosemicarbazones and dioxomolybdenum(VI) complexes.•The complexes show higher binding interactions with CT-DNA than the ligands.•UV absorption titration shows high binding constant values for complexes.•Gel electrophoresis shows differing cleavage patterns for the ligands and complexes.