Oxidative stress mediated cytotoxicity of tellurite in MDA-MB-231 breast cancer cells

Among different metallic compounds, tellurium (Te) was investigated as having anticancer properties. In the current investigation, MDA-MB-231 breast cancer cell line was cultured with or without tellurite to measure live cells by crystal violet staining. TrxR systems can be acted as a leader for ant...

Full description

Saved in:
Bibliographic Details
Published in:Journal of King Saud University. Science 2019-10, Vol.31 (4), p.980-986
Main Authors: Noreen, Ayesha, Rehman, Abdul
Format: Article
Language:English
Subjects:
Breast cancer
Caspase activity
Oxidative stress
Tellurite
Thioredoxin reductases
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Among different metallic compounds, tellurium (Te) was investigated as having anticancer properties. In the current investigation, MDA-MB-231 breast cancer cell line was cultured with or without tellurite to measure live cells by crystal violet staining. TrxR systems can be acted as a leader for anticancer therapy by eliminating cancerous cells through inhibition of TrxR and glutathione reductase (GR). Oxidative stress, generated in higher concentration in tellurite exposed cells, leads to lower the cell reduced environment such as reduced glutathione GSH) and non-protein thiol. Tellurite exposed cells underwent both type of cell death via necrosis and apoptosis. The lateral was slowed as tellurite concentration increased which was measured by caspase-3 activity, thus this finding would be very useful for determination of type and extent of cell death occurred by tellurite via biochemical and morphological ways. By elucidating the role of tellurite in inducing cell death in carcinoma cells by increased production of reactive oxygen species, this method could be employed for the treatment of carcinoma cells.
ISSN:1018-3647
DOI:10.1016/j.jksus.2019.03.001