Effective parameters for stimulation of dissociated cultures using multi-electrode arrays

Electrical stimulation through multi-electrode arrays is used to evoke activity in dissociated cultures of cortical neurons. We study the efficacies of a variety of pulse shapes under voltage control as well as current control, and determine useful parameter ranges that optimize efficacy while preve...

Full description

Saved in:
Bibliographic Details
Published in:Journal of neuroscience methods 2004-09, Vol.138 (1), p.27-37
Main Authors: Wagenaar, Daniel A., Pine, Jerome, Potter, Steve M.
Format: Article
Language:English
Subjects:
2-Amino-5-phosphonovalerate - pharmacology
6-Cyano-7-nitroquinoxaline-2,3-dione - pharmacology
Action Potentials - drug effects
Action Potentials - radiation effects
Animals
Cells, Cultured
Dissociated-culture
Dose-Response Relationship, Radiation
Electric Conductivity
Electric Stimulation - methods
Electrical stimulation
Electricity
Electrodes
Embryo, Mammalian
Excitatory Amino Acid Antagonists - pharmacology
MEA
Membrane Potentials - radiation effects
Multi-electrode array
Neocortex - cytology
Neurons - drug effects
Neurons - physiology
Neurons - radiation effects
Rats
Reaction Time - drug effects
Reaction Time - radiation effects
Reproducibility of Results
Stimulation parameters
Tetrodotoxin - pharmacology
Time Factors
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Electrical stimulation through multi-electrode arrays is used to evoke activity in dissociated cultures of cortical neurons. We study the efficacies of a variety of pulse shapes under voltage control as well as current control, and determine useful parameter ranges that optimize efficacy while preventing damage through electrochemistry. For any pulse shape, stimulation is found to be mediated by negative currents. We find that positive-then-negative biphasic voltage-controlled pulses are more effective than any of the other pulse shapes tested, when compared at the same peak voltage. These results suggest that voltage-control, with its inherent control over limiting electrochemistry, may be advantageous in a wide variety of stimulation scenarios, possibly extending to in-vivo experiments.
ISSN:0165-0270
1872-678X
DOI:10.1016/j.jneumeth.2004.03.005