Simultaneous real-time amperometric measurement of catecholamines and serotonin at carbon fibre ‘dident’ microelectrodes

Amperometry allows real-time measurement of in vivo electrochemical signals, albeit with no capacity to identify the constituents of the signal. In the present study, differential amperometry was used to monitor catecholamine and serotonin (5-HT) simultaneously at the same location. ‘Dident’ carbon...

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Bibliographic Details
Published in:Journal of neuroscience methods 2004-12, Vol.140 (1), p.5-13
Main Authors: Pennington, James M., Millar, Julian, L. Jones, Clinton P., Owesson, Catarina Å., McLaughlin, Daniel P., Stamford, Jonathan A.
Format: Article
Language:English
Subjects:
Animals
Biological Assay - instrumentation
Biological Assay - methods
Brain - anatomy & histology
Brain - metabolism
Brain Chemistry - physiology
Brain slice
Carbon - chemistry
Catecholamines
Catecholamines - analysis
Dual amperometry
Electrophysiology - instrumentation
Electrophysiology - methods
Male
Membrane Potentials - physiology
Mice
Mice, Knockout
Microelectrodes - standards
Monoamine Oxidase - genetics
Neurochemistry - instrumentation
Neurochemistry - methods
Organ Culture Techniques
Rats
Rats, Wistar
Serotonin
Serotonin - analysis
Silver Compounds
Time Factors
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Summary:Amperometry allows real-time measurement of in vivo electrochemical signals, albeit with no capacity to identify the constituents of the signal. In the present study, differential amperometry was used to monitor catecholamine and serotonin (5-HT) simultaneously at the same location. ‘Dident’ carbon fibre microelectrodes (microelectrodes with two working electrodes in a single assembly) were constructed and coated with Nafion to prevent poisoning on contact with brain tissue. One electrode (channel A) was held at +200 mV versus Ag/AgCl to monitor catecholamines selectively. This potential is too low to oxidise 5-HT. The second electrode (channel B), recording faradaic current at +500 mV versus Ag/AgCl, measured the sum of catecholamine and 5-HT oxidation. The 5-HT current component was the difference of channel B minus channel A. With appropriate balancing of the two channels, it is possible to record catecholamines and 5-HT simultaneously at the same dident microelectrode. Examples of measurements in striatum, cortex and locus coeruleus are shown.
ISSN:0165-0270
1872-678X
DOI:10.1016/j.jneumeth.2004.03.023