Green tea catechins inhibit pancreatic phospholipase A2 and intestinal absorption of lipids in ovariectomized rats

• Published in: The Journal of nutritional biochemistry 2006-07, Vol.17 (7), p.492-498
• Main Authors: Wang, S, Noh, S.K, Koo, S.I
• Format: Article
• Language: English
• Subjects: alpha-tocopherol; animal models; antioxidant activity; antioxidants; beverages; Biological and medical sciences; Camellia sinensis; catechin; cholesteryl esters; enzyme inhibition; enzyme inhibitors; epicatechin; Feeding. Feeding behavior; Fundamental and applied biological sciences. Psychology; gallic acid; green tea; in vivo studies; intestinal absorption; lipolysis; ovariectomy; phosphatidylcholines; phospholipase A2; plant extracts; plant pigments; rats; triacylglycerols; Vertebrates: anatomy and physiology, studies on body, several organs or systems; vitamin A
• ISSN: 0955-2863; 1873-4847
• DOI: 10.1016/j.jnutbio.2006.03.004

This study was conducted to examine whether the inhibition of intestinal lipid absorption by green tea is associated with the inhibitory effect of its catechins on pancreatic phospholipase A2 (PLA2). PLA2 activity was assayed by using 1,2-dioleoylphosphatidylcholine (DOPC), porcine pancreatic PLA2 and catechins at varying concentrations (0.075-1.80 μmol/L). The amount of 1-oleoyl-2-hydroxyphosphatidylcholine liberated was determined by HPLC. The percentage of inhibition of PLA2 by catechins at 0.6 μmol increased in the order of (-)-epicatechin (23.3%), (+)-catechin (CAT; 24.8%), (-)-epigallocatechin (25.7%), (-)-epicatechin gallate (39.7%) and (-)-epigallocatechin gallate (EGCG; 64.9%). In an in vivo study, ovariectomized rats with lymph cannula were infused intraduodenally for 8 h with a triolein emulsion containing [dioleoyl-1-14C]-phosphatidylcholine, DOPC, α-tocopherol (αTOH) and retinol (ROH) without (CAT0) or with CAT or EGCG. The lymphatic total 14C-radioactivity was significantly lowered by EGCG (45.5±4.9% dose) compared with CAT (56.2±5.2% dose) and CAT0 (64.7±2.0% dose). The 14C-radioactivity remaining in the small intestinal lumen and cecum was higher in EGCG (24.1% dose) than in CAT (9.5% dose) and CAT0 rats (9.0% dose). Significantly less 14C radioactivity was incorporated into lymph triacylglycerol and cholesteryl ester in EGCG rats. The absorption of αaTOH, used as a marker of extremely hydrophobic lipids, was significantly lower in EGCG (7.8±1.7 μmol) than in CAT (14.4±2.8 μmol) and CAT0 rats (16.8±2.1 μmol). The absorption of ROH was unaffected, whereas oleic acid output was lower in EGCG rats. The results show that EGCG inhibits the intestinal absorption of lipids, which is in part associated with its inhibition of phosphatidylcholine hydrolysis. Data suggest that EGCG may inhibit the absorption of other highly lipophilic organic compounds.