In vitro food–drug interaction study: Which milk component has a decreasing effect on the bioavailability of ciprofloxacin?

The purpose of the present work was developing an in vitro dissolution test to highlight the possible molecular background causing ciprofloxacin (CPFX)–milk interaction. The in vitro dissolution of CPFX from film-coated tablets (Ciprinol ® 500 mg) was examined at different pH values, simulating cert...

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Bibliographic Details
Published in:Journal of pharmaceutical and biomedical analysis 2010-05, Vol.52 (1), p.37-42
Main Authors: Pápai, K., Budai, M., Ludányi, K., Antal, I., Klebovich, I.
Format: Article
Language:English
Subjects:
Analysis
Analytical, structural and metabolic biochemistry
Animals
Anti-Infective Agents - chemistry
Anti-Infective Agents - metabolism
Biological and medical sciences
Biological Availability
Calcium - chemistry
Casein
Caseins - chemistry
Chromatography, High Pressure Liquid
Ciprofloxacin
Ciprofloxacin - chemistry
Ciprofloxacin - metabolism
Food-Drug Interactions
Fundamental and applied biological sciences. Psychology
General pharmacology
Hydrogen-Ion Concentration
In vitro dissolution
Kinetics
Lipids - chemistry
Medical sciences
Milk - adverse effects
Milky medium
Milk–drug interaction study
Pharmacology. Drug treatments
Reproducibility of Results
Solid Phase Extraction
Solubility
Spectrometry, Mass, Electrospray Ionization
Tablets
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Summary:The purpose of the present work was developing an in vitro dissolution test to highlight the possible molecular background causing ciprofloxacin (CPFX)–milk interaction. The in vitro dissolution of CPFX from film-coated tablets (Ciprinol ® 500 mg) was examined at different pH values, simulating certain parts of the gastrointestinal tract, in the presence of water, low-fat milk, casein- or calcium enriched water. In order to determine the amount of dissolved CPFX, solid phase extraction sample preparation followed by high performance liquid chromatography coupled with mass spectrometry was applied. Comparing the dissolution efficiency values in various media, it can be concluded, that casein has a more pronounced effect on the absorbable amount of the antibiotic at each pH value studied, than calcium. In the case of concomitant intake of CPFX film-coated tablet and milk or other dairy products not only the complexation with calcium, but also the adsorption of CPFX on the surface of proteins decreases the absorbable amount of CPFX.
ISSN:0731-7085
1873-264X
DOI:10.1016/j.jpba.2009.12.003