Detection of Multiple Superantigen Genes in Stools of Patients with Kawasaki Disease

Objectives To investigate whether superantigens (SAgs) are involved in the development of Kawasaki disease (KD) by examining SAg genes in the stool of patients with KD. Study design Stool specimens were obtained from 60 patients with KD and 62 age-matched children (36 children with acute illness and...

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Bibliographic Details
Published in:The Journal of pediatrics 2009-08, Vol.155 (2), p.266-270
Main Authors: Suenaga, Tomohiro, MD, Suzuki, Hiroyuki, MD, PhD, Shibuta, Shoichi, MD, Takeuchi, Takashi, MD, Yoshikawa, Norishige, MD, PhD
Format: Article
Language:English
Subjects:
Bacterial Proteins - genetics
Bacterial Toxins - genetics
Biological and medical sciences
Case-Control Studies
Child, Preschool
DNA, Bacterial - isolation & purification
Enterotoxins - genetics
Exotoxins - genetics
Feces - chemistry
Female
General aspects
Humans
Male
Medical sciences
Membrane Proteins - genetics
Mucocutaneous Lymph Node Syndrome - genetics
Mucocutaneous Lymph Node Syndrome - immunology
Pediatrics
Polymerase Chain Reaction
Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. Vasculitis
Superantigens - genetics
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Summary:Objectives To investigate whether superantigens (SAgs) are involved in the development of Kawasaki disease (KD) by examining SAg genes in the stool of patients with KD. Study design Stool specimens were obtained from 60 patients with KD and 62 age-matched children (36 children with acute illness and 26 healthy children). Total DNA was extracted from these stool samples. Using polymerase chain reaction, we examined genes of 5 SAgs: streptococcal pyrogenic exotoxin-A (SPE-A), SPE-C, SPE-G, SPE-J, and toxic shock syndrome toxin-1. Results At least 1 of the 5 SAg genes was detected in 42 (70%) specimens from patients with KD, 14 (38.9%) from the febrile group, and 7 (26.9%) from the healthy group. The detection rate between subjects with and without KD was of at least 1 of the 5 SAg genes ( P < .001), and more than 2 SAg genes were significantly different ( P = .002). Conclusions SAg may be involved in the development of KD; data suggest that multiple SAgs may trigger KD.
ISSN:0022-3476
1097-6833
DOI:10.1016/j.jpeds.2009.03.013